ArticlePlant cell reports2009
Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster).
Article in Plant cell reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 79 citations in OpenAlex.
- A Three-Tier In Vitro Strategy for Accelerated Pine Breeding and Resistance Research Against Pine Wilt Disease.Plants (Basel, Switzerland) · 2026Article
- Laser Microdissection of Woody and Suberized Plant Tissues for RNA-Seq Analysis.Molecular biotechnology · 2023Article
- Current status of the cryopreservation of embryogenic material of woody species.Frontiers in plant science · 2023Review
- Somatic mutations during rapid clonal domestication ofEvolutionary applications · 2022Article
- Clonal fidelity investigation of micropropagated hardened plants of jackfruit tree (Artocarpus heterophyllus L.) with RAPD markers.Journal, genetic engineering & biotechnology · 2022Article
- Article
- Protocol development for somatic embryogenesis, SSR markers and genetic modification of Stipagrostis pennata (Trin.) De Winter.Plant methods · 2021Article
- Review
- Small RNA profiling in Pinus pinaster reveals the transcriptome of developing seeds and highlights differences between zygotic and somatic embryos.Scientific reports · 2019Article
- Genetic homogeneity revealed in micropropagatedPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Analysis of genetic stability of in vitro propagated potato microtubers using DNA markers.Physiology and molecular biology of plants : an international journal of functional plant biology · 2013Article
- Normalizing gene expression by quantitative PCR during somatic embryogenesis in two representative conifer species: Pinus pinaster and Picea abies.Plant cell reports · 2013Article
- Direct and indirect organogenesis of Clivia miniata and assessment of DNA methylation changes in various regenerated plantlets.Plant cell reports · 2012Article
- Tissue culture-induced variation at simple sequence repeats in sorghum (Sorghum bicolor L.) is genotype-dependent and associated with down-regulated expression of a mismatch repair gene, MLH3.Plant cell reports · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Somatic embryogenesis (SE) is a propagation tool of particular interest for accelerating the deployment of new high-performance planting stock in multivarietal forestry. However, genetic conformity in in vitro propagated plants should be assessed as early as possible, especially in long-living trees such as conifers. The main objective of this work was to study such conformity based on genetic stability at simple sequence repeat (SSR) loci during somatic embryogenesis in maritime pine (Pinus pinaster Ait.). Embryogenic cell lines (ECLs) subjected to tissue proliferation during 6, 14 or 22 months, as well as emblings regenerated from several ECLs, were analyzed. Genetic variation at seven SSR loci was detected in ECLs under proliferation conditions for all time points, and in 5 out of 52 emblings recovered from somatic embryos. Three of these five emblings showed an abnormal phenotype consisting mainly of plagiotropism and loss of apical dominance. Despite the variation found in somatic embryogenesis-derived plant material, no correlation was established between genetic stability at the analyzed loci and abnormal embling phenotype, present in 64% of the emblings. The use of microsatellites in this work was efficient for monitoring mutation events during the somatic embryogenesis in P. pinaster. These molecular markers should be useful in the implementation of new breeding and deployment strategies for improved trees using SE.
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