ArticleApplied microbiology and biotechnology2009
Development of a quantitative PCR method to differentiate between viable and nonviable bacteria in environmental water samples.
Article in Applied microbiology and biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 62 citations in OpenAlex.
- Refining microbial biomarker identification in rumen microbiome studies: a viability PCR-based approach.Applied and environmental microbiology · 2025Article
- Utilizing novel Escherichia coli-specific conserved signature proteins for enhanced monitoring of recreational water quality.MicrobiologyOpen · 2024Article
- A comparison of precipitation and filtration-based SARS-CoV-2 recovery methods and the influence of temperature, turbidity, and surfactant load in urban wastewater.The Science of the total environment · 2022Article
- Pre-digest of unprotected DNA by Benzonase improves the representation of living skin bacteria and efficiently depletes host DNA.Microbiome · 2021Article
- Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications - A systematic review.Water research X · 2021Article
- Distribution and Antibiotic Resistance Profiles ofGeoHealth · 2021Article
- Screening and identification of BP100 peptide conjugates active against Xylella fastidiosa using a viability-qPCR method.BMC microbiology · 2020Article
- Molecular Biomarkers and Influential Factors of Denitrification in a Full-Scale Biological Nitrogen Removal Plant.Microorganisms · 2019Article
- Molecular Evaluation of Vitality and Survival Rate of Dormant Kyoho Grape Seedlings: A Step toward Molecular Farming.Plants (Basel, Switzerland) · 2019Article
- Spiking a Silty-Sand Reference Soil with Bacterial DNA: Limits and Pitfalls in the Discrimination of Live and Dead Cells When Applying Ethidium Monoazide (EMA) Treatment.Current microbiology · 2019Article
- Schrödinger's microbes: Tools for distinguishing the living from the dead in microbial ecosystems.Microbiome · 2017Review
- Molecular Viability Testing of UV-Inactivated Bacteria.Applied and environmental microbiology · 2017Article
- Review
- Experimental design for the optimization of propidium monoazide treatment to quantify viable and non-viable bacteria in piggery effluents.BMC microbiology · 2015Article
- Occurrence and Control of Legionella in Recycled Water Systems.Pathogens (Basel, Switzerland) · 2015Review
- CTAB-mediated, single-step preparation of competent Escherichia coli, Bifidobacterium sp. and Kluyveromyces lactis cells.Meta gene · 2014Article
- Use of quantitative real-time PCR for direct detection of serratia marcescens in marine and other aquatic environments.Applied and environmental microbiology · 2014Article
- Molecular viability testing of bacterial pathogens from a complex human sample matrix.PloS one · 2013Article
- Molecular detection of viable bacterial pathogens in water by ratiometric pre-rRNA analysis.Applied and environmental microbiology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ethidium monoazide bromide (EMA) treatment of pure culture and environmental waters at low concentrations (1.0-7.5 microg/ml) indicated effective enumeration of viable and viable but nonculturable Escherichia coli in pure cultures, creek waters, and secondary activated sludge effluent samples by quantitative polymerase chain reaction (qPCR) amplification of the uidA and fliC gene targets at turbidity values < 10 NTU. However, EMA treatment was not effective in primary clarifier and secondary trickling filter effluents where turbidities were > or = 10 NTU. In viable pure cultures, rapidly dividing and senescent cells were most affected by increasing EMA concentrations. Amplification of heat-killed pure bacterial cultures decreased 4 to 6 logs depending on EMA concentration and culture age. The greatest difference was observed in 5-h cultures using 7.5 microg/ml EMA. Turbidity (> or = 100 NTU) in environmental samples inhibited EMA effectiveness on viability discrimination. Enumeration of E. coli in certain wastewaters using EMA-qPCR was similar to culture suggesting that EMA treatment could be incorporated into qPCR assays for the quantification of viable bacteria increasing assay time no more than 30 min. Our results indicate that EMA can be used in routine qPCR assays, but optimum conditions for exposure must be identified for each sample type due to sample matrix effects such as turbidity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.