ArticleMethods in molecular biology (Clifton, N.J.)2009
MudPIT analysis: application to human heart tissue.
Article in Methods in molecular biology (Clifton, N.J.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of this Field.Proteomes · 2020Review
- Quantitative proteomic analysis reveals metabolic alterations, calcium dysregulation, and increased expression of extracellular matrix proteins in laminin α2 chain-deficient muscle.Molecular & cellular proteomics : MCP · 2014Article
- Application of activity-based protein profiling to study enzyme function in adipocytes.Methods in enzymology · 2014Article
- Proteomic profiling of the influence of iron availability on Cryptococcus gattii.Journal of proteome research · 2012Article
- Seasonal liver protein differences in a hibernator revealed by quantitative proteomics using whole animal isotopic labeling.Comparative biochemistry and physiology. Part D, Genomics & proteomics · 2011Article
- Advancing alcohol biomarkers research.Alcoholism, clinical and experimental research · 2010Article
- Proteomics and mass spectrometry: what have we learned about the heart?Current cardiology reviews · 2010Article
- Proteomics: challenges, techniques and possibilities to overcome biological sample complexity.Human genomics and proteomics : HGP · 2009Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Although two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) has been used as the standard proteomic approach for separating proteins in a complex mixture, this technique has many drawbacks. These include a limited molecular mass range, poor separation of highly acidic or basic proteins, and exclusion of the majority of membrane proteins from analysis. Considering the important functions of many membrane proteins, such as receptors, ion transporters, signal transducers, and cell adhesion proteins, it is increasingly important that these proteins are not excluded during the global proteomic analysis of cellular systems. Multidimensional Protein Identification Technology (MudPIT) offers a gel-free alternative to 2D-PAGE for the analysis of both membrane and soluble proteins.The goal of this chapter is to provide detailed methods for using MudPIT to profile both membrane and soluble proteins in complex unfractionated samples. Methods discussed will include tissue homogenization, sample preparation, MudPIT, data analysis, and an application for the analysis of unfractionated total tissue homogenate from human heart.
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