ArticleThe Journal of cell biology2009
Reversible binding and rapid diffusion of proteins in complex with inositol lipids serves to coordinate free movement with spatial information.
Article in The Journal of cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed.
- Nanoscale clustering and dynamics of phosphatidylinositol 4,5-bisphosphate in an immune cell model.Biophysical journal · 2025Article
- Pck2 association with the plasma membrane and efficient response of the cell integrity pathway require regulation of PI4P homeostasis by exomer.Open biology · 2024Article
- Probing and imaging phospholipid dynamics in live cells.Life metabolism · 2024Review
- PI(4,5)P2 diffuses freely in the plasma membrane even within high-density effector protein complexes.The Journal of cell biology · 2023Article
- Going with the membrane flow: the impact of polarized secretion on bulk plasma membrane flows.The FEBS journal · 2023Review
- PI(4,5)P2: signaling the plasma membrane.The Biochemical journal · 2022Article
- Computational framework for single-cell spatiotemporal dynamics of optogenetic membrane recruitment.Cell reports methods · 2022Article
- Sequential compound fusion and kiss-and-run mediate exo- and endocytosis in excitable cells.Science advances · 2022Article
- Quantitative FRET Microscopy Reveals a Crucial Role of Cytoskeleton in Promoting PI(4,5)PInternational journal of molecular sciences · 2021Article
- Cell patterning by secretion-induced plasma membrane flows.Science advances · 2021Article
- Membrane Nanoscopic Organization of D2L Dopamine Receptor Probed by Quantum Dot Tracking.Membranes · 2021Article
- Exomer Is Part of a Hub Where Polarized Secretion and Ionic Stress Connect.Frontiers in microbiology · 2021Article
- A polybasic domain in aPKC mediates Par6-dependent control of membrane targeting and kinase activity.The Journal of cell biology · 2020Article
- Super-Resolution Localisation of Nuclear PI(4)P and Identification of Its Interacting Proteome.Cells · 2020Article
- Imaging FCS delineates subtle heterogeneity in plasma membranes of resting mast cells.Molecular biology of the cell · 2020Article
- RAP GTPases and platelet integrin signaling.Platelets · 2019Review
- Visualization of expanding fusion pores in secretory cells.The Journal of general physiology · 2018Review
- In vivo measurement of afferent activity with axon-specific calcium imaging.Nature neuroscience · 2018Article
- Genetically encoded lipid biosensors.Molecular biology of the cell · 2018Article
- Membrane Diffusion Occurs by Continuous-Time Random Walk Sustained by Vesicular Trafficking.Biophysical journal · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Polyphosphoinositol lipids convey spatial information partly by their interactions with cellular proteins within defined domains. However, these interactions are prevented when the lipids' head groups are masked by the recruitment of cytosolic effector proteins, whereas these effectors must also have sufficient mobility to maximize functional interactions. To investigate quantitatively how these conflicting functional needs are optimized, we used different fluorescence recovery after photobleaching techniques to investigate inositol lipid-effector protein kinetics in terms of the real-time dissociation from, and diffusion within, the plasma membrane. We find that the protein-lipid complexes retain a relatively rapid ( approximately 0.1-1 microm(2)/s) diffusion coefficient in the membrane, likely dominated by protein-protein interactions, but the limited time scale (seconds) of these complexes, dictated principally by lipid-protein interactions, limits their range of action to a few microns. Moreover, our data reveal that GAP1(IP4BP), a protein that binds PtdIns(4,5)P(2) and PtdIns(3,4,5)P(3) in vitro with similar affinity, is able to "read" PtdIns(3,4,5)P(3) signals in terms of an elongated residence time at the membrane.
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