ArticleThe Journal of physiology2009
The spatial pattern of exocytosis and post-exocytic mobility of synaptopHluorin in mouse motor nerve terminals.
Article in The Journal of physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Presynaptic Mitochondria Communicate With Release Sites for Spatio-Temporal Regulation of Exocytosis at the Motor Nerve Terminal.Frontiers in synaptic neuroscience · 2022Article
- Presynaptic mechanisms controlling calcium-triggered transmitter release at the neuromuscular junction.Current opinion in physiology · 2018Article
- Impact of spatiotemporal calcium dynamics within presynaptic active zones on synaptic delay at the frog neuromuscular junction.Journal of neurophysiology · 2018Article
- Coupling the Structural and Functional Assembly of Synaptic Release Sites.Frontiers in neuroanatomy · 2018Review
- Homeostatic synaptic plasticity at the neuromuscular junction in myasthenia gravis.Annals of the New York Academy of Sciences · 2018Review
- The Active and Periactive Zone Organization and the Functional Properties of Small and Large Synapses.Frontiers in synaptic neuroscience · 2016Article
- Different dynamin blockers interfere with distinct phases of synaptic endocytosis during stimulation in motoneurones.The Journal of physiology · 2015Article
- Evaluation of a novel calcium channel agonist for therapeutic potential in Lambert-Eaton myasthenic syndrome.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Are unreliable release mechanisms conserved from NMJ to CNS?Trends in neurosciences · 2013Review
- Live imaging of bulk endocytosis in frog motor nerve terminals using FM dyes.Journal of neurophysiology · 2011Article
- Active zones and the readily releasable pool of synaptic vesicles at the neuromuscular junction of the mouse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Frequency-dependent modes of synaptic vesicle endocytosis and exocytosis at adult mouse neuromuscular junctions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- The origins and evolution of freeze-etch electron microscopy.Journal of electron microscopy · 2011Review
- Motor neuron-specific overexpression of the presynaptic choline transporter: impact on motor endurance and evoked muscle activity.Neuroscience · 2010Article
- Preferred sites of exocytosis and endocytosis colocalize during high- but not lower-frequency stimulation in mouse motor nerve terminals.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We monitored the spatial distribution of exo- and endocytosis at 37 degrees C in mouse motor nerve terminals expressing synaptopHluorin (spH), confirming and extending earlier work at room temperature, which had revealed fluorescent 'hot spots' appearing in repeatable locations during tetanic stimulation. We also tested whether hot spots appeared during mild stimulation. Averaged responses from single shocks showed a clear fluorescence jump, but revealed no sign of hot spots; instead, fluorescence rose uniformly across the terminal. Only after 5-25 stimuli given at high frequency did hot spots appear, suggesting a novel initiation mechanism. Experiments showed that about half of the surface spH molecules were mobile, and that spH movement occurred out of hot spots, demonstrating their origin as exocytic sources, not endocytic sinks. Taken together, our results suggest that synaptic vesicles exocytose equally throughout the terminal with mild stimulation, but preferentially exocytose at specific, repeatable locations during tetanic stimulation.
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