ArticleNucleic acids research2009
Generation of single-chain LAGLIDADG homing endonucleases from native homodimeric precursor proteins.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- Comprehensive analysis of insertion sequences within rRNA genes of CPR bacteria and biochemical characterization of a homing endonuclease encoded by these sequences.Journal of bacteriology · 2024Article
- Prokaryotic Argonaute Proteins as a Tool for Biotechnology.Molecular biology · 2022Article
- Points of View on the Tools for Genome/Gene Editing.International journal of molecular sciences · 2021Review
- Highlighting of a LAGLIDADG and a Zing Finger Motifs Located in the pUL56 Sequence Crucial for HCMV Replication.Viruses · 2019Article
- New Human Chromosomal Sites with "Safe Harbor" Potential for Targeted Transgene Insertion.Human gene therapy · 2019Article
- Engineering altered protein-DNA recognition specificity.Nucleic acids research · 2018Review
- The Structural Basis of Asymmetry in DNA Binding and Cleavage as Exhibited by the I-SmaMI LAGLIDADG Meganuclease.Journal of molecular biology · 2016Article
- Article
- Engineering domain fusion chimeras from I-OnuI family LAGLIDADG homing endonucleases.Nucleic acids research · 2012Article
- LAHEDES: the LAGLIDADG homing endonuclease database and engineering server.Nucleic acids research · 2012Article
- Chemical and biological approaches to improve the efficiency of homologous recombination in human cells mediated by artificial restriction DNA cutter.Nucleic acids research · 2012Article
- Expanding LAGLIDADG endonuclease scaffold diversity by rapidly surveying evolutionary sequence space.Nucleic acids research · 2012Article
- Comprehensive homing endonuclease target site specificity profiling reveals evolutionary constraints and enables genome engineering applications.Nucleic acids research · 2012Article
- Article
- Comprehensive computational design of mCreI homing endonuclease cleavage specificity for genome engineering.Nucleic acids research · 2011Article
- Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy.Current gene therapy · 2011Review
- Article
- Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus.Nucleic acids research · 2011Article
- Computational reprogramming of homing endonuclease specificity at multiple adjacent base pairs.Nucleic acids research · 2010Article
- Generation of redesigned homing endonucleases comprising DNA-binding domains derived from two different scaffolds.Nucleic acids research · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Homing endonucleases (HEs) cut long DNA target sites with high specificity to initiate and target the lateral transfer of mobile introns or inteins. This high site specificity of HEs makes them attractive reagents for gene targeting to promote DNA modification or repair. We have generated several hundred catalytically active, monomerized versions of the well-characterized homodimeric I-CreI and I-MsoI LAGLIDADG family homing endonuclease (LHE) proteins. Representative monomerized I-CreI and I-MsoI proteins (collectively termed mCreIs or mMsoIs) were characterized in detail by using a combination of biochemical, biophysical and structural approaches. We also demonstrated that both mCreI and mMsoI proteins can promote cleavage-dependent recombination in human cells. The use of single chain LHEs should simplify gene modification and targeting by requiring the expression of a single small protein in cells, rather than the coordinate expression of two separate protein coding genes as is required when using engineered heterodimeric zinc finger or homing endonuclease proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.