ArticleNucleic acids research2009
A self-cleaving DNA enzyme modified with amines, guanidines and imidazoles operates independently of divalent metal cations (M2+).
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 133 citations in OpenAlex.
- Dynamism and evolvability in nucleic acid enzymes.Nature communications · 2026Article
- Chemically modified DNA aptamers and DNAzymes for expanded functional capabilities.Chemical science · 2026Review
- Probing three-dimensional cyclooctatetraene for nucleobase modification in aptamer selection.Communications chemistry · 2025Article
- Sequencing a DNA analog composed of artificial bases.Nature communications · 2025Article
- Polymerase Synthesis of Hypermodified DNA Displaying a Combination of Thiol, Hydroxyl, Carboxylate, and Imidazole Functional Groups in the Major Groove.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Zwitterionic DNA: enzymatic synthesis of hypermodified DNA bearing four different cationic substituents at all four nucleobases.Nucleic acids research · 2025Article
- Template-dependent DNA ligation for the synthesis of modified oligonucleotides.Nature communications · 2024Article
- Superanionic DNA: enzymatic synthesis of hypermodified DNA bearing four different anionic substituents at all four nucleobases.Nucleic acids research · 2023Article
- A modular XNAzyme cleaves long, structured RNAs under physiological conditions and enables allele-specific gene silencing.Nature chemistry · 2022Article
- Dependence of click-SELEX performance on the nature and average number of modified nucleotides.RSC chemical biology · 2022Article
- Nonenzymatic assembly of active chimeric ribozymes from aminoacylated RNA oligonucleotides.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- In vitro Selection of Chemically Modified DNAzymes.ChemistryOpen · 2020Review
- Dynamic changes in DNA populations revealed by split-combine selection.Chemical science · 2020Article
- Molecular Features and Metal Ions That Influence 10-23 DNAzyme Activity.Molecules (Basel, Switzerland) · 2020Review
- Site-specific functionalization with amino, guanidinium, and imidazolyl groups enabling the activation of 10-23 DNAzyme.RSC advances · 2020Article
- Tethered imidazole mediated duplex stabilization and its potential for aptamer stabilization.Nucleic acids research · 2018Article
- Review
- Evolution of sequence-defined highly functionalized nucleic acid polymers.Nature chemistry · 2018Article
- A densely modified MChemical science · 2018Article
- Theranostic DNAzymes.Theranostics · 2017Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The selection of modified DNAzymes represents an important endeavor in expanding the chemical and catalytic properties of catalytic nucleic acids. Few examples of such exist and to date, there is no example where three different modified bases have been simultaneously incorporated for catalytic activity. Herein, dCTP, dATP and dUTP bearing, respectively, a cationic amine, an imidazole and a cationic guanidine, were enzymatically polymerized on a DNA template for the selection of a highly functionalized DNAzyme, called DNAzyme 9-86, that catalyzed (M(2+))-independent self-cleavage under physiological conditions at a single ribo(cytosine)phosphodiester linkage with a rate constant of (0.134 +/- 0.026) min(-1). A pH rate profile analysis revealed pK(a)'s of 7.4 and 8.1, consistent with both general acid and base catalysis. The presence of guanidinium cations permits cleavage at significantly higher temperatures than previously observed for DNAzymes with only amines and imidazoles. Qualitatively, DNAzyme 9-86 presents an unprecedented ensemble of synthetic functionalities while quantitatively it expresses one of the highest reported values for any self-cleaving nucleic acid when investigated under M(2+)-free conditions at 37 degrees C.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.