ArticleWorld journal of gastroenterology2009
Application of stool-PCR test for diagnosis of Helicobacter pylori infection in children.
Article in World journal of gastroenterology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Non-invasive diagnostic tests for Helicobacter pylori infection.The Cochrane database of systematic reviews · 2018Pooled it
- Article
- Effect of concurrent infection of Helicobacter pylori with Toxoplasma gondii infection on gastric pathology.BMC infectious diseases · 2024Article
- An investigative study on the zoonotic potential of Helicobacter pylori.BMC veterinary research · 2023Article
- Usefulness of Molecular Methods forInternational journal of molecular sciences · 2022Article
- Molecular characterization ofSaudi medical journal · 2022Article
- An exploratory study on the presence of Helicobacter heilmannii and Helicobacter billis in the feces of companion dogs.Veterinary medicine and science · 2022Article
- Identification of Factors Associated with Biofilm Formation Ability in the Clinical Isolates ofIranian journal of biotechnology · 2017Article
- Article
- Association of hp1181 and hp1184 Genes With the Active Efflux Phenotype in Multidrug-Resistant Isolates of Helicobacter pylori.Jundishapur journal of microbiology · 2016Article
- Analysis of Genomic Diversity among Helicobacter pylori Strains Isolated from Iranian Children by Pulsed Field Gel Electrophoresis.Iranian journal of pediatrics · 2014Article
- Molecular oncology testing in resource-limited settings.The Journal of molecular diagnostics : JMD · 2014Review
- Evaluation of an Iranian Home-made Helicobacter pylori Stool Antigen ELISA Kit.Jundishapur journal of microbiology · 2014Article
- Usefulness of a Helicobacter pylori stool antigen test for diagnosing H. pylori infected C57BL/6 mice.Laboratory animal research · 2013Article
- Bacterial urease and its role in long-lasting human diseases.Current protein & peptide science · 2012Review
- Non-invasive genotyping of Helicobacter pylori cagA, vacA, and hopQ from asymptomatic children.Helicobacter · 2012Article
- Culture Method and PCR for the Detection of Helicobacter pylori in Drinking Water in Basrah Governorate Iraq.Gastroenterology research and practice · 2012Article
- Helicobacter pylori diagnostic tests in children: review of the literature from 1999 to 2009.European journal of pediatrics · 2010Review
- Study of biofilm formation in C57Bl/6J mice by clinical isolates of Helicobacter pylori.Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology AssociationArticle
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo evaluate the usefulness of stool-PCR test for diagnosis of Helicobacter pylori (H pylori) infection in pediatric populations.
methodsBased on endoscopic features (including nodular gastritis, erosive duodenitis and ulcer) and/or a positive rapid urease test (RUT) obtained during endoscopy, 28 children from a group of children admitted to the Children's Medical Center of Tehran for persistent upper gastrointestinal problems were selected to compare biopsy-based tests with stool-PCR. Their gastric activity and bacterial density were graded by the updated Sydney system, and their first stool after endoscopy was stored at -70 degrees C. Biopsies were cultured on modified campy-blood agar plates and identified by gram-staining, biochemical tests, and PCR. Two methods of phenol-chloroform and boiling were used for DNA extraction from H pylori isolates. Isolation of DNA from stool was performed using a stool DNA extraction kit (Bioneer Inc, Korea). PCR was performed using primers for detection of vacA, cagA, and 16srRNA genes in both isolates and stool.
resultsSixteen out of 28 child patients (57%) were classified as H pylori positive by biopsy-based tests, of which 11 (39%) were also positive by stool-PCR. Sensitivity and specificity of stool-PCR was 62.5% and 92.3% respectively. H pylori was observed in histological sections for 10 out of 11 stool-positive patients. Association was observed between higher score of H pylori in histology and positivity of stool-PCR. Also association was observed between the more severe form of gastritis and a positive stool-PCR.
conclusionAssociation between higher score of H pylori in histology and a positive stool-PCR make it a very useful test for detection of H pylori active infection in children. We also suggest that a simple stool-PCR method can be a useful test for detection of H pylori virulence genes in stool.
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