ArticleJournal of medical virology2009
Detection of human bocavirus in respiratory, fecal, and blood samples by real-time PCR.
Article in Journal of medical virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed.
- Virus detection and its association with symptoms during influenza-like illness in a sample of healthy adults enrolled in a randomised controlled vaccine trial.Influenza and other respiratory viruses · 2013Trial
- Tracking the occurrence and fate of human bocavirus 2 in municipal wastewater from a small city.Molecular biology reports · 2026Article
- Human Bocavirus Circulating in Patients with Acute Gastroenteritis in Taiwan, 2018-2022.Viruses · 2024Article
- Development of RPA-Cas12a-fluorescence assay for rapid and reliable detection of human bocavirus 1.Animal models and experimental medicine · 2024Article
- Clinical manifestations and outcome of viral acute lower respiratory infection in hospitalised children in Myanmar.BMC infectious diseases · 2022Article
- Beyond Cytomegalovirus and Epstein-Barr Virus: a Review of Viruses Composing the Blood Virome of Solid Organ Transplant and Hematopoietic Stem Cell Transplant Recipients.Clinical microbiology reviews · 2020Review
- Impact of RSV Coinfection on Human Bocavirus in Children with Acute Respiratory Infections.Journal of tropical pediatrics · 2019Article
- Comparative Viral Sampling in the Sinonasal Passages; Different Viruses at Different Sites.Frontiers in cellular and infection microbiology · 2018Article
- Prevalence of Human Bocavirus in Africa and Other Developing Countries between 2005 and 2016: A Potential Emerging Viral Pathogen for Diarrhea.Journal of tropical medicine · 2018Review
- Detection of viruses in weekly stool specimens collected during the first 2 years of life: A pilot study of five healthy Australian infants in the rotavirus vaccine era.Journal of medical virology · 2017Article
- Human Parvoviruses.Clinical microbiology reviews · 2017Review
- Frequent Detection and Genetic Diversity of Human Bocavirus in Urban Sewage Samples.Food and environmental virology · 2016Article
- Human bocavirus: Current knowledge and future challenges.World journal of gastroenterology · 2016Review
- Detection of respiratory viruses and bacteria in children using a twenty-two target reverse-transcription real-time PCR (RT-qPCR) panel.World journal of pediatrics : WJP · 2016Article
- Mapping Antigenic Epitopes on the Human Bocavirus Capsid.Journal of virology · 2016Article
- Prevalence and Phylogenetic Analysis of Human Bocaviruses 1-4 in Pediatric Patients with Various Infectious Diseases.PloS one · 2016Article
- Structure of an enteric pathogen, bovine parvovirus.Journal of virology · 2015Article
- Adenovirus species C is associated with chronic suppurative lung diseases in children.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2014Article
- Article
- Nasal swab samples and real-time polymerase chain reaction assays in community-based, longitudinal studies of respiratory viruses: the importance of sample integrity and quality control.BMC infectious diseases · 2014Observational
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human bocavirus (HBoV) has been detected worldwide in respiratory samples. Two real-time PCR assays, targeting the non-structural protein (NP-1) and viral protein (VP-1) genes, were designed and validated to detect HBoV in patients with respiratory disease, gastroenteritis, or systemic illness. Sensitivity of the NP-1 and VP-1 assays were equal to the conventional PCR assay previously described by Allander et al. [2005: Proc Natl Acad Sci USA 102: 12891-12896] being 100%, and giving specificity of 94% and 93%, respectively. There was no cross-reaction identified with unrelated respiratory agents, or to human DNA. The limits of detection were 10 copies of genomic DNA equivalents per reaction for both assays. The assays were used to screen three different sample populations, combined nose, and throat swabs (n = 96) from children with acute respiratory disease, fecal samples (n = 375) from adults, and children with gastroenteritis and whole blood (n = 229) collected from 31 immunocompromised children taken over an 18-month period. In total 17 (18%) respiratory samples and 18 (4.8%) fecal samples were identified as having HBoV present. Of the pediatric whole blood specimens investigated, HBoV was detected in six (2.6%) samples from four patients. In summary, two real-time PCR assays targeting different genes were designed and validated for use as screening methods for the detection of HBoV. HBoV was found in three different specimen types: parent-collected combined nose-throat swabs, fecal samples collected from symptomatic individuals and whole blood from immunocompromised children.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.