ArticleEukaryotic cell2009
Patterns of gene-specific and total transcriptional activity during the Plasmodium falciparum intraerythrocytic developmental cycle.
Article in Eukaryotic cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 75 citations in OpenAlex.
- High-resolution map of the Plasmodium falciparum genome reveals MORC/ApiAP2-mediated links between distant, functionally related genes.Nature microbiology · 2025Article
- Malian children infected with Plasmodium ovale and Plasmodium falciparum display very similar gene expression profiles.PLoS neglected tropical diseases · 2023Article
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- Determination of the Stage Composition ofmSystems · 2022Article
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- The RNA structurome in the asexual blood stages of malaria pathogenRNA biology · 2021Article
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- Histone modifications associated with gene expression and genome accessibility are dynamically enriched at Plasmodium falciparum regulatory sequences.Epigenetics & chromatin · 2020Article
- Plasmodium vivax transcriptomes reveal stage-specific chloroquine response and differential regulation of male and female gametocytes.Nature communications · 2019Article
- Genome-wide real-time in vivo transcriptional dynamics during Plasmodium falciparum blood-stage development.Nature communications · 2018Article
- Chromatin Accessibility-Based Characterization of the Gene Regulatory Network Underlying Plasmodium falciparum Blood-Stage Development.Cell host & microbe · 2018Article
- Single-cell RNA sequencing reveals a signature of sexual commitment in malaria parasites.Nature · 2017Article
- Nascent RNA sequencing reveals mechanisms of gene regulation in the human malaria parasite Plasmodium falciparum.Nucleic acids research · 2017Article
- Single-molecule analysis reveals that DNA replication dynamics vary across the course of schizogony in the malaria parasite Plasmodium falciparum.Scientific reports · 2017Article
- Cluster analysis ofF1000Research · 2016Article
- Plasmodium parasites mount an arrest response to dihydroartemisinin, as revealed by whole transcriptome shotgun sequencing (RNA-seq) and microarray study.BMC genomics · 2015Article
- Triaging informative cis-regulatory elements for the combinatorial control of temporal gene expression during Plasmodium falciparum intraerythrocytic development.Parasites & vectors · 2015Article
- Genome-wide analysis in Plasmodium falciparum reveals early and late phases of RNA polymerase II occupancy during the infectious cycle.BMC genomics · 2014Article
- Homopolymer tract organization in the human malarial parasite Plasmodium falciparum and related Apicomplexan parasites.BMC genomics · 2014Article
- MRI-localized biopsies reveal subtype-specific differences in molecular and cellular composition at the margins of glioblastoma.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The relationships among gene regulatory mechanisms in the malaria parasite Plasmodium falciparum throughout its asexual intraerythrocytic developmental cycle (IDC) remain poorly understood. To investigate the level and nature of transcriptional activity and its role in controlling gene expression during the IDC, we performed nuclear run-on on whole-transcriptome samples from time points throughout the IDC and found a peak in RNA polymerase II-dependent transcriptional activity related to both the number of nuclei per parasite and variable transcriptional activity per nucleus over time. These differential total transcriptional activity levels allowed the calculation of the absolute transcriptional activities of individual genes from gene-specific nuclear run-on hybridization data. For half of the genes analyzed, sense-strand transcriptional activity peaked at the same time point as total activity. The antisense strands of several genes were substantially transcribed. Comparison of the transcriptional activity of the sense strand of each gene to its steady-state RNA abundance across the time points assayed revealed both correlations and discrepancies, implying transcriptional and posttranscriptional regulation, respectively. Our results demonstrate that such comparisons can effectively indicate gene regulatory mechanisms in P. falciparum and suggest that genes with diverse transcriptional activity levels and patterns combine to produce total transcriptional activity levels tied to parasite development during the IDC.
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