ArticleApplied and environmental microbiology2009
Isolation and identification of cellulose-binding proteins from sheep rumen contents.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 46 citations in OpenAlex.
- Exploration of Multi-Source Lignocellulose-Degrading Microbial Resources and Bioaugmentation Strategies: Implications for Rumen Efficiency.Animals : an open access journal from MDPI · 2025Review
- - Invited Review - Understanding the functionality of the rumen microbiota: searching for better opportunities for rumen microbial manipulation.Animal bioscience · 2024Article
- Comparative and functional analyses of fecal microbiome in Asian elephants.Antonie van Leeuwenhoek · 2022Article
- Deciphering the unique cellulose degradation mechanism of the ruminal bacterium Fibrobacter succinogenes S85.Scientific reports · 2019Article
- Meta-proteomics of rumen microbiota indicates niche compartmentalisation and functional dominance in a limited number of metabolic pathways between abundant bacteria.Scientific reports · 2018Article
- Comparative characterization of bacterial communities in geese fed all-grass or high-grain diets.PloS one · 2017Article
- Long-Term Enrichment on Cellulose or Xylan Causes Functional and Taxonomic Convergence of Microbial Communities from Anaerobic Digesters.Applied and environmental microbiology · 2015Article
- Metaproteomics of complex microbial communities in biogas plants.Microbial biotechnology · 2015Review
- Article
- A Phylogenomic Analysis of the Bacterial Phylum Fibrobacteres.Frontiers in microbiology · 2015Article
- The Role of Mass Spectrometry in the "Omics" Era.Current organic chemistry · 2013Article
- Metagenome Analysis of Protein Domain Collocation within Cellulase Genes of Goat Rumen Microbes.Asian-Australasian journal of animal sciences · 2013Article
- Exploring mixed microbial community functioning: recent advances in metaproteomics.FEMS microbiology ecology · 2012Review
- Metagenomic analyses: past and future trends.Applied and environmental microbiology · 2011Review
- Marine metagenomics: new tools for the study and exploitation of marine microbial metabolism.Marine drugs · 2010Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To extend our understanding of the mechanisms of plant cell wall degradation in the rumen, cellulose-binding proteins (CBPs) from the contents of a sheep rumen were directly isolated and identified using a metaproteomics approach. The rumen CBPs were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and some CBPs revealed endoglucanase activities toward carboxymethyl cellulose. Using mass spectrometry analyses, four CBPs were identified and annotated as known proteins from the predominant rumen cellulolytic bacterium Fibrobacter succinogenes: tetratricopeptide repeat domain protein, OmpA family protein, fibro-slime domain protein, and cellulose-binding endoglucanase F (EGF). Another CBP was identified as the cellulosomal glycosyl hydrolase family 6 exoglucanase, Cel6A, of Piromyces equi. F. succinogenes cells expressing EGF were found to be major members of the bacterial community on the surface or at the inner surface of hay stems by immunohistochemical analyses using anti-EGF antibody. The finding that four of the five CBPs isolated and identified from sheep rumen contents were from F. succinogenes indicates that F. succinogenes is significantly involved in cellulose degradation in the rumen.
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