ArticleApplied and environmental microbiology2009
Bacterial diversity analysis of Huanglongbing pathogen-infected citrus, using PhyloChip arrays and 16S rRNA gene clone library sequencing.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 141 citations in OpenAlex.
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- Peptide nucleic acid (PNA) clamps reduce amplification of host chloroplast and mitochondria rRNA gene sequences and increase detected diversity in 16S rRNA gene profiling analysis of oak-associated microbiota.Environmental microbiome · 2025Article
- Dynamic changes in the endophytic bacterial communities of huanglongbing-affected periwinkle leaves.Frontiers in microbiology · 2025Article
- Deciphering Phyllomicrobiome of Cauliflower Leaf: Revelation by Metagenomic and Microbiological Analysis of Tolerant and Susceptible Genotypes Against Black Rot Disease.Current microbiology · 2024Article
- Endophytes and Plant Extracts as Potential Antimicrobial Agents againstMicroorganisms · 2023Article
- Microbiome diversity, composition and assembly in a California citrus orchard.Frontiers in microbiology · 2023Article
- Citrus Huanglongbing detection and semi-quantification of the carbohydrate concentration based on micro-FTIR spectroscopy.Analytical and bioanalytical chemistry · 2022Article
- The Upregulated Expression of the CitrusInternational journal of molecular sciences · 2022Article
- Infection and distribution of Candidatus Liberibacter asiaticus in citrus plants and psyllid vectors at the cellular level.Microbial biotechnology · 2022Article
- Article
- Bioprospecting Microbiome for Soil and Plant Health Management Amidst Huanglongbing Threat in Citrus: A Review.Frontiers in plant science · 2022Review
- Defeating Huanglongbing PathogenFrontiers in plant science · 2021Article
- Core endophyte communities of different citrus varieties from citrus growing regions in China.Scientific reports · 2020Article
- Cold Plasma Treatment of Sunflower Seeds Modulates Plant-Associated Microbiome and Stimulates Root and Lateral Organ Growth.Frontiers in plant science · 2020Article
- Asymptomatic Diagnosis of Huanglongbing Disease Using Metalloporphyrin Functionalized Single-Walled Carbon Nanotubes Sensor Arrays.Frontiers in chemistry · 2020Article
- Huanglongbing Control: Perhaps the End of the Beginning.Microbial ecology · 2018Review
- Studies of Microbiota Dynamics Reveals Association of "International journal of molecular sciences · 2018Article
- A Lethal Fungus Infects the Chinese White Wax Scale Insect and Causes Dramatic Changes in the Host Microbiota.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bacterial diversity associated with citrus leaf midribs was characterized for citrus groves that contained the Huanglongbing (HLB) pathogen, which has yet to be cultivated in vitro. We employed a combination of high-density phylogenetic 16S rRNA gene microarrays and 16S rRNA gene clone library sequencing to determine the microbial community composition for symptomatic and asymptomatic citrus midribs. Our results revealed that citrus leaf midribs can support a diversity of microbes. PhyloChip analysis indicated that 47 orders of bacteria in 15 phyla were present in the citrus leaf midribs, while 20 orders in 8 phyla were observed with the cloning and sequencing method. PhyloChip arrays indicated that nine taxa were significantly more abundant in symptomatic midribs than in asymptomatic midribs. "Candidatus Liberibacter asiaticus" was detected at a very low level in asymptomatic plants but was over 200 times more abundant in symptomatic plants. The PhyloChip analysis results were further verified by sequencing 16S rRNA gene clone libraries, which indicated the dominance of "Candidatus Liberibacter asiaticus" in symptomatic leaves. These data implicate "Candidatus Liberibacter asiaticus" as the pathogen responsible for HLB disease.
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