ArticleNucleic acids research2009
RAD50 and NBS1 form a stable complex functional in DNA binding and tethering.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 62 citations in OpenAlex.
- Structural basis for DNA break sensing by human MRE11-RAD50-NBS1 and its regulation by telomeric factor TRF2.Nature communications · 2025Article
- Cohesin complex oligomerization maintains end-tethering at DNA double-strand breaks.Nature cell biology · 2025Article
- DNA damage remodels the MITF interactome to increase melanoma genomic instability.Genes & development · 2024Article
- Modeling cancer genomic data in yeast reveals selection against ATM function during tumorigenesis.PLoS genetics · 2020Article
- Functional and structural insights into the MRX/MRN complex, a key player in recognition and repair of DNA double-strand breaks.Computational and structural biotechnology journal · 2020Review
- Focus-ING on DNA Integrity: Implication of ING Proteins in Cell Cycle Regulation and DNA Repair Modulation.Cancers · 2019Review
- Activation of Tel1The Journal of biological chemistry · 2019Article
- Review
- Interdependent and separable functions ofProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
- Eukaryotic resectosomes: A single-molecule perspective.Progress in biophysics and molecular biology · 2017Review
- Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair.Journal of visualized experiments : JoVE · 2017Article
- Architectural plasticity of human BRCA2-RAD51 complexes in DNA break repair.Nucleic acids research · 2017Article
- Eukaryotic Rad50 functions as a rod-shaped dimer.Nature structural & molecular biology · 2017Article
- The Mre11-Nbs1 Interface Is Essential for Viability and Tumor Suppression.Cell reports · 2017Article
- Impact of the MRN Complex on Adeno-Associated Virus Integration and Replication during Coinfection with Herpes Simplex Virus 1.Journal of virology · 2015Article
- Interdependence of the rad50 hook and globular domain functions.Molecular cell · 2015Article
- SFMetrics: an analysis tool for scanning force microscopy images of biomolecules.BMC bioinformatics · 2015Article
- Productive replication of human papillomavirus 31 requires DNA repair factor Nbs1.Journal of virology · 2014Article
- Caffeine suppresses homologous recombination through interference with RAD51-mediated joint molecule formation.Nucleic acids research · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The RAD50/MRE11/NBS1 protein complex (RMN) plays an essential role during the early steps of DNA double-strand break (DSB) repair by homologous recombination. Previous data suggest that one important role for RMN in DSB repair is to provide a link between DNA ends. The striking architecture of the complex, a globular domain from which two extended coiled coils protrude, is essential for this function. Due to its DNA-binding activity, ability to form dimers and interact with both RAD50 and NBS1, MRE11 is considered to be crucial for formation and function of RMN. Here, we show the successful expression and purification of a stable complex containing only RAD50 and NBS1 (RN). The characteristic architecture of the complex was not affected by absence of MRE11. Although MRE11 is a DNA-binding protein it was not required for DNA binding per se or DNA-tethering activity of the complex. The stoichiometry of NBS1 in RMN and RN complexes was estimated by SFM-based volume analysis. These data show that in vitro, R, M and N form a variety of stable complexes with variable subunit composition and stoichiometry, which may be physiologically relevant in different aspects of RMN function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.