ArticleNucleic acids research2009
Sensing peptide-oligonucleotide interactions by a two-color fluorescence label: application to the HIV-1 nucleocapsid protein.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Multi-parametric sensing by multi-channel molecular fluorescent probes based on excited state intramolecular proton transfer and charge transfer processes.BBA advances · 2023Article
- Article
- Electronic transitions and ESIPT kinetics of the thienyl-3-hydroxychromone nucleobase surrogate in DNA duplexes: a DFT/MD-TDDFT study.RSC advances · 2020Article
- Ab initio study of polar and non-polar aprotic solvents effects on some 3-hydroxychromones and 3-hydroxyquinolones derivatives.Journal of molecular modeling · 2018Article
- An uracil-linked hydroxyflavone probe for the recognition of ATP.Beilstein journal of organic chemistry · 2018Article
- The HIV-1 nucleocapsid protein recruits negatively charged lipids to ensure its optimal binding to lipid membranes.Journal of virology · 2015Article
- Fluorescence characteristics of some flavones probes in different micellar media.Journal of fluorescence · 2014Article
- A phenyl-thiadiazolylidene-amine derivative ejects zinc from retroviral nucleocapsid zinc fingers and inactivates HIV virions.Retrovirology · 2012Article
- Intrinsic nucleic acid dynamics modulates HIV-1 nucleocapsid protein binding to its targets.PloS one · 2012Article
- Structural determinants of TAR RNA-DNA annealing in the absence and presence of HIV-1 nucleocapsid protein.Nucleic acids research · 2011Article
- Specific implications of the HIV-1 nucleocapsid zinc fingers in the annealing of the primer binding site complementary sequences during the obligatory plus strand transfer.Nucleic acids research · 2011Article
- The relationship between HIV-1 genome RNA dimerization, virion maturation and infectivity.Nucleic acids research · 2011Article
- The concept of λ-ratiometry in fluorescence sensing and imaging.Journal of fluorescence · 2010Review
- Monitoring protein interactions and dynamics with solvatochromic fluorophores.Trends in biotechnology · 2010Review
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Authors and funding
4 authors.
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Abstract
We present a new methodology for site-specific sensing of peptide-oligonucleotide (ODN) interactions using a solvatochromic fluorescent label based on 3-hydroxychromone (3HC). This label was covalently attached to the N-terminus of a peptide corresponding to the zinc finger domain of the HIV-1 nucleocapsid protein (NC). On interaction with target ODNs, the labeled peptide shows strong changes in the ratio of its two emission bands, indicating an enhanced screening of the 3HC fluorophore from the bulk water by the ODN bases. Remarkably, this two-color response depends on the ODN sequence and correlates with the 3D structure of the corresponding complexes, suggesting that the 3HC label monitors the peptide-ODN interactions site-specifically. By measuring the two-color ratio, we were also able to determine the peptide-ODN-binding parameters and distinguish multiple binding sites in ODNs, which is rather difficult using other fluorescence methods. Moreover, this method was found to be more sensitive than the commonly used steady-state fluorescence anisotropy, especially in the case of small ODNs. The described methodology could become a new universal tool for investigating peptide-ODN interactions.
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