ArticleBiochemical and biophysical research communications2009
Lack of coupling between membrane stretching and pannexin-1 hemichannels.
Article in Biochemical and biophysical research communications, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Arterial Myocyte Pannexin 1 Channel Controls Vascular Reactivity in Diabetic Hyperglycemia.Circulation research · 2025Article
- A physiologic rise in cytoplasmic calcium ion signal increases pannexin1 channel activity via a C-terminus phosphorylation by CaMKII.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Stretch-Induced Activation of Pannexin 1 Channels Can Be Prevented by PKA-Dependent Phosphorylation.International journal of molecular sciences · 2020Article
- The two faces of pannexins: new roles in inflammation and repair.Journal of inflammation research · 2018Review
- Inhibitors of connexin and pannexin channels as potential therapeutics.Pharmacology & therapeutics · 2017Review
- Astrocytes in the optic nerve head express putative mechanosensitive channels.Molecular vision · 2015Article
- The pannexins: past and present.Frontiers in physiology · 2014Review
- Connexin and pannexin hemichannels in brain glial cells: properties, pharmacology, and roles.Frontiers in pharmacology · 2013Article
- Pannexin 1 forms an anion-selective channel.Pflugers Archiv : European journal of physiology · 2012Article
- A co-operative regulation of neuronal excitability by UNC-7 innexin and NCA/NALCN leak channel.Molecular brain · 2011Article
- Extracellular osmolarity modulates G protein-coupled receptor-dependent ATP release from 1321N1 astrocytoma cells.American journal of physiology. Cell physiology · 2010Article
- Functional interactions between P2X4 and P2X7 receptors from mouse salivary epithelia.The Journal of physiology · 2009Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We investigated whether pannexin-1, a carbenoxolone-sensitive hemichannel activated in erythrocytes by swelling, could be activated by swelling stress and contribute to swelling-activated chloride currents (I(Cl,swell)) in HEK-293 cells. We used ethidium bromide uptake as an index of pannexin-1 activation and I(C,swell) activation as an index of plasma membrane stretching. I(Cl,swell) activated by a hypotonic solution was reversible inhibited by carbenoxolone (IC(50) 98+/-5 microM). However, the hypotonic solution that activated I(Cl,swell) did not induce ethidium bromide uptake indicating that pannexin-1 was not activated by cell swelling. The mimetic peptide (10)panx1, a pannexin-1 antagonist, did not affect I(Cl,swell) activation but completely inhibited the ATP-induced ethidium bromide uptake coupled to P2X(7) receptors activation. We conclude that carbenoxolone directly inhibited I(Cl,swell) independent of pannexin-1 and that pannexin-1 hemichannels are not activated by swelling in HEK-293 cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.