ArticleThe Journal of antimicrobial chemotherapy2009
Roles of lipooligosaccharide and capsular polysaccharide in antimicrobial resistance and natural transformation of Campylobacter jejuni.
Article in The Journal of antimicrobial chemotherapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 50 citations in OpenAlex.
- Cas9 modulatesMicrobiology (Reading, England) · 2025Article
- Identification of Outer Membrane Vesicles as a New Vehicle Mediating Antibiotic Resistance Gene Transfer in Campylobacter.Journal of extracellular vesicles · 2025Article
- ClpB enhances thermotolerance inMicrobiology spectrum · 2025Article
- Temporal dynamics of gene expression during the development ofMicrobial genomics · 2025Article
- Discovery of a new volcanic soil material, "Akahoya," as an adsorbent for bacterial and viral pathogens and its application to environmental purification.Applied and environmental microbiology · 2024Article
- Phylogenetic Association and Genetic Factors in Cold Stress Tolerance in Campylobacter jejuni.Microbiology spectrum · 2022Article
- Barriers to genetic manipulation of Enterococci: Current Approaches and Future Directions.FEMS microbiology reviews · 2022Review
- Deciphering the genetic network and programmed regulation of antimicrobial resistance in bacterial pathogens.Frontiers in cellular and infection microbiology · 2022Review
- An Updated Classification System and Review of the Lipooligosaccharide Biosynthesis Gene Locus inFrontiers in microbiology · 2020Review
- Article
- A Newly Discovered Drug Resistance GeneInfection and drug resistance · 2019Article
- Genetic exchanges are more frequent in bacteria encoding capsules.PLoS genetics · 2018Article
- Key Role of Capsular Polysaccharide in the Induction of Systemic Infection and Abortion by Hypervirulent Campylobacter jejuni.Infection and immunity · 2017Article
- Identification of potential Campylobacter jejuni genes involved in biofilm formation by EZ-Tn5 Transposome mutagenesis.BMC research notes · 2017Article
- Draft Genome Sequences of Three Multiantibiotic-Resistant Campylobacter jejuni Strains (2865, 2868, and 2871) Isolated from Poultry at Retail Outlets in Malaysia.Genome announcements · 2016Article
- Contribution of surface polysaccharides to the resistance of Campylobacter jejuni to antimicrobial phenolic compounds.The Journal of antibiotics · 2015Article
- Antibiotic modulation of capsular exopolysaccharide and virulence in Acinetobacter baumannii.PLoS pathogens · 2015Article
- Antimicrobial resistance in the food chain: a review.International journal of environmental research and public health · 2013Review
- Antibody to a conserved antigenic target is protective against diverse prokaryotic and eukaryotic pathogens.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Resistance mechanisms in Campylobacter jejuni.Virulence · 2013Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
objectivesThis study is aimed to determine the role of capsular polysaccharide (CPS) and lipooligosaccharide (LOS) in modulating antimicrobial resistance and natural transformation of Campylobacter jejuni, an important food-borne human pathogen.
methodsA series of C. jejuni mutants, which are defective in either CPS or LOS or both, were constructed. The antimicrobial susceptibility, bacterial surface hydrophobicity, natural transformation frequency and DNA binding and uptake were measured and compared between the mutants and the wild-type strain.
resultsTruncation of LOS greatly reduced (8-fold) the intrinsic resistance of C. jejuni to erythromycin, a key antibiotic used for treating human campylobacteriosis, while the loss of CPS did not result in significant changes in the susceptibility to antimicrobial agents. Notably, mutation of LOS also significantly increased (>16-fold) the susceptibility to erythromycin in C. jejuni mutants carrying the A2074G mutation in 23S rRNA. The increased susceptibility to erythromycin in the LOS mutant was probably due to enhanced permeability to this antibiotic, because the LOS mutation rendered the surface of C. jejuni more hydrophobic. Loss of CPS and truncation of LOS increased the transformation frequency by 4- and 25-fold, respectively, and mutation of both CPS and LOS resulted in a 97-fold increase in the transformation frequency. Consistent with the increased transformation frequencies, the CPS and LOS mutants showed enhanced rates of DNA uptake.
conclusionsThese results demonstrate that the surface polysaccharides in C. jejuni contribute to the resistance to erythromycin, a clinically important antibiotic, but restrict natural transformation.
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Registered trials
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