ArticleMicrobes and infection2009
Enhanced antibody responses to Plasmodium falciparum Pfs28 induced in mice by conjugation to ExoProtein A of Pseudomonas aeruginosa with an improved procedure.
Article in Microbes and infection, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Diversity and selection analyses identify transmission-blocking antigens as the optimal vaccine candidates in Plasmodium falciparum.EBioMedicine · 2024Article
- Diversity and selection analyses identify transmission-blocking antigens as the optimal vaccine candidates inmedRxiv : the preprint server for health sciences · 2024Article
- Structural analysis of Plasmodium falciparum ookinete surface antigen Pfs28 relevant for malaria vaccine design.Scientific reports · 2022Article
- Assessment of Crosslinkers between Peptide Antigen and Carrier Protein for Fusion Peptide-Directed Vaccines against HIV-1.Vaccines · 2022Article
- Molecular interactions between parasite and mosquito during midgut invasion as targets to block malaria transmission.NPJ vaccines · 2021Review
- Transmission-Blocking Vaccines: Harnessing Herd Immunity for Malaria Elimination.Expert review of vaccines · 2021Review
- Comparison of carrier proteins to conjugate malaria transmission blocking vaccine antigens, Pfs25 and Pfs230.Vaccine · 2020Article
- Host cell protein quantification of an optimized purification method by mass spectrometry.Journal of pharmaceutical and biomedical analysis · 2019Article
- Outer membrane protein complex as a carrier for malaria transmission blocking antigen Pfs230.NPJ vaccines · 2019Article
- Assessment of Antibodies Induced by Multivalent Transmission-Blocking Malaria Vaccines.Frontiers in immunology · 2017Article
- Protein-protein conjugate nanoparticles for malaria antigen delivery and enhanced immunogenicity.PloS one · 2017Article
- Strategies & recent development of transmission-blocking vaccines againstThe Indian journal of medical research · 2016Review
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- Particle-based platforms for malaria vaccines.Vaccine · 2015Review
- Determination of protein concentration for protein-protein conjugates using ultraviolet absorption.Journal of immunological methods · 2013Article
- Comparative Genomics and Systems Biology of Malaria ParasitesCurrent bioinformatics · 2012Article
- The biology of sexual development of Plasmodium: the design and implementation of transmission-blocking strategies.Malaria journal · 2012Article
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Corrections and comments
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Authors and funding
14 authors.
Funding
Abstract
In this paper we report our efforts to enhance the immunogenicity of Pfs28, a transmission blocking vaccine candidate of Plasmodium falciparum, using a strategy of chemical conjugation. With an improved procedure, Pfs28 was covalently coupled to the mutant and non-toxic ExoProtein A of Pseudomonas aeruginosa by the reaction between thiolated antigen and maleimide modified carrier protein. The optimized process resulted in a higher antigen-carrier conjugation ratio, and the conjugation product could be purified using single-step size-exclusion chromatography. A significant increase in immunogenicity measured by ELISA was observed in mice immunized with conjugated Pfs28 as compared to unconjugated Pfs28.
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