ArticleRNA (New York, N.Y.)2009
Genetic and structural analysis of base substitutions in the central pseudoknot of Thermus thermophilus 16S ribosomal RNA.
Article in RNA (New York, N.Y.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- A Survey of Spontaneous Antibiotic-Resistant Mutants of the Halophilic, Thermophilic BacteriumAntibiotics (Basel, Switzerland) · 2021Article
- Article
- Resistance to UV Irradiation Caused by Inactivation ofJournal of bacteriology · 2018Article
- Engineering the genome of Thermus thermophilus using a counterselectable marker.Journal of bacteriology · 2015Article
- The DEAH-box helicase Dhr1 dissociates U3 from the pre-rRNA to promote formation of the central pseudoknot.PLoS biology · 2015Article
- Phenotypic interactions among mutations in a Thermus thermophilus 16S rRNA gene detected with genetic selections and experimental evolution.Journal of bacteriology · 2014Article
- Structural analysis of base substitutions in Thermus thermophilus 16S rRNA conferring streptomycin resistance.Antimicrobial agents and chemotherapy · 2014Article
- Article
- The central role of protein S12 in organizing the structure of the decoding site of the ribosome.RNA (New York, N.Y.) · 2013Article
- Tools for characterizing bacterial protein synthesis inhibitors.Antimicrobial agents and chemotherapy · 2013Article
- Reorganization of an intersubunit bridge induced by disparate 16S ribosomal ambiguity mutations mimics an EF-Tu-bound state.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Structural and functional studies of the Thermus thermophilus 16S rRNA methyltransferase RsmG.RNA (New York, N.Y.) · 2009Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Characterization of base substitutions in rRNAs has provided important insights into the mechanism of protein synthesis. Knowledge of the structural effects of such alterations is limited, and could be greatly expanded with the development of a genetic system based on an organism amenable to both genetics and structural biology. Here, we describe the genetic analysis of base substitutions in 16S ribosomal RNA of the extreme thermophile Thermus thermophilus, and an analysis of the conformational effects of these substitutions by structure probing with base-specific modifying agents. Gene replacement methods were used to construct a derivative of strain HB8 carrying a single 16S rRNA gene, allowing the isolation of spontaneous streptomycin-resistant mutants and subsequent genetic mapping of mutations by recombination. The residues altered to give streptomycin resistance reside within the central pseudoknot structure of 16S rRNA comprised of helices 1 and 27, and participate in the U13-U20-A915 base triple, the G21-A914 type II sheared G-A base pair, or the G885-C912 Watson-Crick base pair closing helix 27. Substitutions at any of the three residues engaged in the base triple were found to confer resistance. Results from structure probing of the pseudoknot are consistent with perturbation of RNA conformation by these substitutions, potentially explaining their streptomycin-resistance phenotypes.
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