ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2009
Agonist occupancy is essential for forward trafficking of AMPA receptors.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- Distinct Regulation of Early Trafficking of the NMDA Receptors by the Ligand-Binding Domains of the GluN1 and GluN2A Subunits.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Synaptic Dysfunction by Mutations inBrain sciences · 2022Article
- The Extracellular Domains of GluN Subunits Play an Essential Role in Processing NMDA Receptors in the ER.Frontiers in neuroscience · 2021Review
- Cognate Ligand Chaperoning: a Novel Mechanism for the Post-translational Regulation of Neurotransmitter Receptor Biogenesis.Frontiers in cellular neuroscience · 2017Article
- Mechanistic Insight into NMDA Receptor Dysregulation by Rare Variants in the GluN2A and GluN2B Agonist Binding Domains.American journal of human genetics · 2016Article
- A parallel panning scheme used for selection of a GluA4-specific Fab targeting the ligand-binding domain.International journal of biological macromolecules · 2016Article
- Aggregation Limits Surface Expression of Homomeric GluA3 Receptors.The Journal of biological chemistry · 2016Article
- Neuronal gamma-aminobutyric acid (GABA) type A receptors undergo cognate ligand chaperoning in the endoplasmic reticulum by endogenous GABA.Frontiers in cellular neuroscience · 2015Article
- Assembly of AMPA receptors: mechanisms and regulation.The Journal of physiology · 2015Review
- The N-terminal domain modulates α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor desensitization.The Journal of biological chemistry · 2014Article
- Pharmacological chaperoning: a primer on mechanism and pharmacology.Pharmacological research · 2014Review
- GPR37 protein trafficking to the plasma membrane regulated by prosaposin and GM1 gangliosides promotes cell viability.The Journal of biological chemistry · 2014Article
- Orchestration of secretory protein folding by ER chaperones.Biochimica et biophysica acta · 2013Review
- Agonist binding to the GluK5 subunit is sufficient for functional surface expression of heteromeric GluK2/GluK5 kainate receptors.Cellular and molecular neurobiology · 2013Article
- Glutamate binding to the GluN2B subunit controls surface trafficking of N-methyl-D-aspartate (NMDA) receptors.The Journal of biological chemistry · 2012Article
- Cornichons modify channel properties of recombinant and glial AMPA receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Behind the curtain: cellular mechanisms for allosteric modulation of calcium-sensing receptors.British journal of pharmacology · 2012Review
- The expanding social network of ionotropic glutamate receptors: TARPs and other transmembrane auxiliary subunits.Neuron · 2011Review
- The biochemistry, ultrastructure, and subunit assembly mechanism of AMPA receptors.Molecular neurobiology · 2010Review
- Ligand-binding domain determines endoplasmic reticulum exit of AMPA receptors.The Journal of biological chemistry · 2010Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regulated trafficking of AMPA receptors to cell surface and to synapses is an important determinant of neuronal excitability. In the present study, we have addressed the role of agonist binding and desensitization in the early trafficking of glutamate receptor-D (GluR-D) AMPA receptors. Analysis of point-mutated GluR-D receptors, via electrophysiology and immunofluorescence, revealed that agonist-binding activity is essential for efficient delivery to cell surface in transfected cell lines and in neurons. Cotransfection with stargazin could fully rescue the surface expression of nonbinding mutant receptors in cell lines, indicating that stargazin is able to interact with and promote exit of AMPA receptors from endoplasmic reticulum (ER) independently of agonist binding. Secretion of separately expressed ligand-binding domain constructs showed a similar dependency of agonist binding to that observed with full-length GluR-D, supporting the idea that glutamate-induced closure of the binding site cleft is registered by ER quality control as a necessary priming step for transport competence. In contrast to agonist binding, the ability of the receptor to undergo desensitization had only a minor influence on trafficking. Our results are consistent with the hypothesis that AMPA receptors are synthesized as intrinsically unstable molecules, which require glutamate binding for structural stability and for transport-competence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.