ArticleJournal of clinical microbiology2009
Modified general primer PCR system for sensitive detection of multiple types of oncogenic human papillomavirus.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
69 citing papers in PubMed, 1 synthesis or guideline pooled it, 148 citations in OpenAlex.
- Detection of Human Papillomaviruses in the Nasopharynx of Breastfed Infants: New Findings and Meta-Analysis.Viruses · 2020Pooled it
- Controlled trial of cervical cancer screening frequency among human-papillomavirus-vaccinated women.International journal of cancer · 2026Trial
- Randomised trial on treatment of vaginal high-grade squamous intraepithelial lesion: Self-administered vaginal imiquimod and laser vaporisation.International journal of cancer · 2025Trial
- Long term duration of protective effect for HPV negative women: follow-up of primary HPV screening randomised controlled trial.BMJ (Clinical research ed.) · 2014Trial
- Human papillomavirus 16 E6 oncoprotein promotes up-regulation of RNA-binding protein Sam68 in head and neck cancer.Frontiers in microbiology · 2026Article
- Development of a Microfluidic Point-of-Care Platform for HPV Detection Based on Helicase-Dependent Amplification.Tropical medicine and infectious disease · 2025Article
- The prevalence of nine human herpesviruses in urogenital samples from young women in Finland.BMC infectious diseases · 2025Article
- Identification of circulating human papillomavirus types through high-throughput sequencing of Canadian municipal and institutional wastewater samples.Applied and environmental microbiology · 2025Article
- Predictors for regression and progression of actively surveilled cervical intraepithelial neoplasia grade 2: A prospective cohort study.Acta obstetricia et gynecologica Scandinavica · 2025Observational
- Cervical cancer microbiome analysis: comparing HPV 16 and 18 with other HPV types.Scientific reports · 2024Article
- Increased prevalence of human papillomavirus in fresh tissue from penile cancers compared to non-malignant penile samples: a case-control study.BMC cancer · 2022Article
- Concurrent Infection With Multiple Human Papillomavirus Types Among Unvaccinated and Vaccinated 17-Year-Old Norwegian Girls.The Journal of infectious diseases · 2022Article
- Co-testing in cervical screening among 40- to 42-year-old women is unreasonable.Acta obstetricia et gynecologica Scandinavica · 2022Article
- Article
- Tonsillar Cancer with High CD8Cancers · 2021Article
- Molecular and immunophenotypic characterization of anal squamous cell carcinoma reveals distinct clinicopathologic groups associated with HPV and TP53 mutation status.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2021Article
- Differing Age-Specific Cervical Cancer Incidence Between Different Types of Human Papillomavirus: Implications for Predicting the Impact of Elimination Programs.American journal of epidemiology · 2021Article
- Distribution of HPV Genotypes Differs Depending on Behavioural Factors among Young Women.Microorganisms · 2021Article
- Penile intraepithelial neoplasia, penile cancer precursors and human papillomavirus prevalence in symptomatic preputium: a cross-sectional study of 351 circumcised men in Sweden.BJU international · 2021Article
- The Effect of Indian Fig Fruit Extract on Human Papilloma Virus containing Cervical Cancer Cells (HeLa) by Decreasing the HPV18 L1 Gene Load.Asian Pacific journal of cancer prevention : APJCP · 2021Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human papillomavirus (HPV) infection is a necessary cause of cervical cancer and cervical dysplasia. Accurate and sensitive genotyping of multiple oncogenic HPVs is essential for a multitude of both clinical and research uses. We developed a modified general primer (MGP) PCR system with five forward and five reverse consensus primers. The MGP system was compared to the classical HPV general primer system GP5+/6+ using a proficiency panel with HPV plasmid dilutions as well as cervical samples from 592 women with low-grade cytological abnormalities. The reference method (GP5+/6+) had the desirable high sensitivity (five copies/PCR) for five oncogenic HPV types (HPV type 16 [HPV-16], HPV-18, HPV-56, HPV-59, and HPV-66). The MGP system was able to detect all 14 oncogenic HPV types at five copies/PCR. In the clinical samples, the MGP system detected a significantly higher proportion of women with more than two concomitant HPV infections than did the GP5+/6+ system (102/592 women compared to 42/592 women). MGP detected a significantly greater number of infections with HPV-16, -18, -31, -33, -35, -39, -42, -43, -45, -51, -52, -56, -58, and -70 than did GP5+/6+. In summary, the MGP system primers allow a more sensitive amplification of most of the HPV types that are established as oncogenic and had an improved ability to detect multiple concomitant HPV infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.