ArticleJournal of clinical microbiology2009
Internal control for nucleic acid testing based on the use of purified Bacillus atrophaeus subsp. globigii spores.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Development and validation of a high-throughput qPCR platform for the detection of soil-transmitted helminth infections.PLoS neglected tropical diseases · 2025Article
- Comparison of rectal swabs and fecal samples for the detection ofMicrobiology spectrum · 2024Article
- Construction and application of a heterogeneous quality control library for the Xpert MTB/RIF assay in tuberculosis diagnosis.Frontiers in cellular and infection microbiology · 2023Article
- Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry with Time-of-Flight Peak Analysis for Rapid and Accurate Detection of Group B Streptococcus in Pregnant Women.Microbiology spectrum · 2022Article
- BCIG-SMAC medium and PMA-qPCR for differential detection of viableIranian journal of microbiology · 2021Article
- A novel method for rapid and sensitive detection of viable Escherichia coli cells using UV-induced PMA-coupled quantitative PCR.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- A Sensitive and Accurate Recombinase Polymerase Amplification Assay for Detection of the Primary Bacterial Pathogens Causing Bovine Respiratory Disease.Frontiers in veterinary science · 2020Article
- A Standard Method To Inactivate Bacillus anthracis Spores to Sterility via Gamma Irradiation.Applied and environmental microbiology · 2018Article
- Molecular method for detection of total coliforms in drinking water samples.Applied and environmental microbiology · 2014Article
- Real-time polymerase chain reaction for microbiological diagnosis of parapneumonic effusions in Canadian children.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2014Article
- Cross-institute evaluations of inhibitor-resistant PCR reagents for direct testing of aerosol and blood samples containing biological warfare agent DNA.Applied and environmental microbiology · 2014Article
- Abilities of the mCP Agar method and CRENAME alpha toxin-specific real-time PCR assay to detect Clostridium perfringens spores in drinking water.Applied and environmental microbiology · 2013Article
- Rapid filtration separation-based sample preparation method for Bacillus spores in powdery and environmental matrices.Applied and environmental microbiology · 2012Article
- The potential of TaqMan Array Cards for detection of multiple biological agents by real-time PCR.PloS one · 2012Article
- Rapid concentration and molecular enrichment approach for sensitive detection of Escherichia coli and Shigella species in potable water samples.Applied and environmental microbiology · 2011Article
- Comparison of eight methods for the extraction of Bacillus atrophaeus spore DNA from eleven common interferents and a common swab.PloS one · 2011Article
- Toward Automatic Label-Free Whispering Gallery Modes Biodetection with a Quantum Dot-Coated Microsphere Population.Nanoscale research letters · 2010Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Commonly used internal controls (ICs) to monitor the efficiency of nucleic acid testing (NAT) assays do not allow verification of nucleic acid extraction efficiency. Since microbial cells are often difficult to lyse, it is important to ensure that nucleic acids are efficiently extracted from any target organism. For this purpose, we developed a cellular IC based on the use of nonpathogenic Bacillus spores. Purified Bacillus atrophaeus subsp. globigii (referred to hereafter as simply B. atrophaeus) spores were added to vaginal and anal samples, which were then subjected to rapid DNA extraction and subsequent PCR amplification. The proof of concept of this cellular IC was made through the use of both manual and automated DNA extraction methods, using vaginal or anal samples spiked with B. atrophaeus spores, combined with a multiplex real-time PCR assay for the specific detection of group B streptococci (GBS) and B. atrophaeus. The performance of the cellular IC was compared to that of a standard IC plasmid added to PCRs. Approximately 500 B. atrophaeus spores per PCR was found to be optimal since this did not interfere significantly with GBS detection for either DNA extraction method and yielded reproducible amplification and/or detection of B. atrophaeus genomic DNA serving as an IC template. Performance of the cellular IC was comparable to that of the standard IC. This novel IC system using nonpathogenic and hard-to-lyse B. atrophaeus spores allowed validation of both the DNA extraction procedure and the amplification and detection process. Use of a spore-based control also provides a universal control for microbial cell lysis.
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