ArticleJournal of clinical microbiology2009
Biofilm formation by Staphylococcus haemolyticus.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
86 citing papers in PubMed, 223 citations in OpenAlex.
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- Coagulase-Negative Staphylococci (CoNS): An Emerging Threat, Antimicrobial Resistance, and Health Concern.International journal of microbiology · 2026Review
- Article
- A narrative overview of Staphylococcus haemolyticus: resistance traits, virulence factors, and health impact.Molecular biology reports · 2025Review
- Genomic and phenotypic characterization of multidrug-resistantMicrobiology spectrum · 2025Article
- Heterogeneity of Biofilm Formation AmongAntibiotics (Basel, Switzerland) · 2025Article
- Virulence factors and biofilm forming ability of Staphyloccoccus species isolated from skeletal lesions of broiler chickens.Scientific reports · 2025Article
- The Gut Microbiota's Role in Neurological, Psychiatric, and Neurodevelopmental Disorders.Nutrients · 2024Review
- Pathogenic potential of meat-borne coagulase negative staphylococci strains from slaughterhouse to fork.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Characterization of virulence factors and antimicrobial resistance in Staphylococcus spp. isolated from clinical samples.Folia microbiologica · 2024Article
- Sticking together: independent evolution of biofilm formation in different species of staphylococci has occurred multiple times via different pathways.BMC genomics · 2024Article
- A Retrospective Descriptive Study ofAnimals : an open access journal from MDPI · 2024Article
- Whole Genome Sequence Analysis of Two Oxacillin-Resistant andInfection and drug resistance · 2024Article
- Antibacterial activity and antibacterial mechanism of flavaspidic acid BB against Staphylococcus haemelyticus.BMC microbiology · 2023Article
- Clonal Spreading of ST42International journal of molecular sciences · 2023Article
- Using next generation antimicrobials to target the mechanisms of infection.npj antimicrobials and resistance · 2023Review
- Trends in Occurrence and Phenotypic Resistance of Coagulase-Negative Staphylococci (CoNS) Found in Human Blood in the Northern Netherlands between 2013 and 2019.Microorganisms · 2022Article
- The Emergence of a Multidrug-Resistant and Pathogenic ST42 Lineage of Staphylococcus haemolyticus from a Hospital in China.Microbiology spectrum · 2022Article
- Comparative profiling of agr locus, virulence, and biofilm-production genes of human and ovine non-aureus staphylococci.BMC veterinary research · 2022Article
26 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infections due to coagulase-negative staphylococci (CoNS) most frequently occur after the implantation of medical devices and are attributed to the biofilm-forming potential of CoNS. Staphylococcus haemolyticus is the second most frequently isolated CoNS from patients with hospital-acquired infections. There is only limited knowledge of the nature of S. haemolyticus biofilms. The aim of this study was to characterize S. haemolyticus biofilm formation. We analyzed the biofilm-forming capacities of 72 clinical S. haemolyticus isolates. A detachment assay with NaIO(4), proteinase K, or DNase was used to determine the main biofilm components. Biofilm-associated genes, including the ica operon, were analyzed by PCR, and the gene products were sequenced. Confocal laser scanning microscopy (CLSM) was used to elucidate the biofilm structure. Fifty-three isolates (74%) produced biofilms after growth in Trypticase soy broth (TSB) with glucose, but only 22 (31%) produced biofilms after growth in TSB with NaCl. It was necessary to dissolve the biofilm in ethanol-acetone to measure the optical density of the full biofilm mass. DNase, proteinase K, and NaIO(4) caused biofilm detachment for 100%, 98%, and 38% of the isolates, respectively. icaRADBC and polysaccharide intercellular adhesin (PIA) production were found in only two isolates. CLSM indicated that the biofilm structure of S. haemolyticus clearly differs from that of S. epidermidis. We conclude that biofilm formation is a common phenotype in clinical S. haemolyticus isolates. In contrast to S. epidermidis, proteins and extracellular DNA are of functional relevance for biofilm accumulation, whereas PIA plays only a minor role. The induction of biofilm formation and determination of the biofilm mass also needed to be optimized for S. haemolyticus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.