ArticleJournal of clinical microbiology2009
Molecular typing of Mycobacterium bovis strains isolated in Italy from 2000 to 2006 and evaluation of variable-number tandem repeats for geographically optimized genotyping.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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50 citing papers in PubMed.
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- Bovine tuberculosis source attribution using decision tree analysis: breakdown investigations in Italy (2022-2023).Frontiers in veterinary science · 2025Article
- InsideFrontiers in microbiology · 2024Article
- Genetic Diversity and Population Structure ofPathogens (Basel, Switzerland) · 2023Article
- First Insight into Diversity of Minisatellite Loci inDiagnostics (Basel, Switzerland) · 2023Article
- Geo-epidemiology of animal tuberculosis andFrontiers in microbiology · 2023Article
- Evidence, Challenges, and Knowledge Gaps Regarding Latent Tuberculosis in Animals.Microorganisms · 2022Review
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- Validation of a Novel Diagnostic Approach Combining the VersaTREK™ System for Recovery and Real-Time PCR for the Identification ofMicroorganisms · 2021Article
- Phylogenetic relationships investigation of Mycobacterium caprae strains from sympatric wild boar and goats based on whole genome sequencing.Transboundary and emerging diseases · 2021Article
- Single-nucleotide polymorphism-based epidemiological analysis of KoreanJournal of veterinary science · 2021Article
- Genotypic Characterization ofFrontiers in veterinary science · 2021Article
- Genetic evaluation of Mycobacteriumbovis isolates with MIRU-VNTR and spoligotypingTurkish journal of medical sciences · 2020Article
- Long-term molecular surveillance provides clues on a cattle origin for Mycobacterium bovis in Portugal.Scientific reports · 2020Article
- Infection byItalian journal of food safety · 2020Article
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14 authors.
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Abstract
Spoligotyping and exact tandem repeat (ETR) analysis of Mycobacterium bovis and M. caprae isolated strains has been routinely carried out in Italy since 2000 to obtain a database of genetic profiles and support traditional epidemiological investigations. In this study, we characterized 1,503 M. bovis and 57 M. caprae isolates obtained from 2000 to 2006 in 747 cattle herds mainly located in northern Italy. We identified 81 spoligotypes and 113 ETR profiles, while the combination of spoligotyping/ETR analysis differentiated 228 genotypes, with genotypic diversity indices of 0.70 (spoligotyping), 0.94 (ETR-A to -E typing), and 0.97 (spoligotyping/ETR-A to -E typing), respectively. Despite the high degree of resolution obtained, the spoligotyping/ETR methods were not discriminative enough in the case of genotypes characterized by the combination of SB0120, the predominant spoligotype in Italy, with the most common ETR profiles. To obtain a more informative subset of typing loci, 24 mycobacterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR) markers were evaluated by analyzing a panel of 100 epidemiologically unrelated SB0120 isolates. The panel was differentiated into 89 profiles with an overall genotypic diversity of 0.987 that could be also achieved by using a minimal group of 13 loci: ETR-A, -B, and -E; MIRU 26 and 40; and VNTR 2163a, 2163b, 3155, 1612, 4052, 1895, 3232, and 3336. The allelic diversity index and the stability of single loci was evaluated to provide the most discriminative genotyping method for locally prevalent strains.
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