ArticlePlant physiology2009
Experimental evidence for ascorbate-dependent electron transport in leaves with inactive oxygen-evolving complexes.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 114 citations in OpenAlex.
- The commonly used electron donor 2,6-dichlorophenolindophenol also serves as an efficient electron acceptor for Photosystem I.Photosynthesis research · 2025Article
- Thermal stability changes of photosynthesis during osmotic and salt stress in wheat varieties cultivated in Central Europe and Mediterranean North Africa.Photosynthetica · 2025Article
- Chloroplastic ascorbate modifies plant metabolism and may act as a metabolite signal regardless of oxidative stress.Plant physiology · 2024Article
- The ascorbate-glutathione cycle coming of age.Journal of experimental botany · 2024Review
- Multiple Physiological and Biochemical Functions of Ascorbic Acid in Plant Growth, Development, and Abiotic Stress Response.International journal of molecular sciences · 2024Review
- Genome-wide association study unveils ascorbate regulation by PAS/LOV PROTEIN during high light acclimation.Plant physiology · 2023Article
- The lifetime of the oxygen-evolving complex subunit PSBO depends on light intensity and carbon availability in Chlamydomonas.Plant, cell & environment · 2023Article
- The Functions of Chloroplastic Ascorbate in Vascular Plants and Algae.International journal of molecular sciences · 2023Review
- Effect of Prolonged Photoperiod on Light-Dependent Photosynthetic Reactions inInternational journal of molecular sciences · 2022Article
- Effective Mechanisms for Improving Seed Oil Production in Pennycress (Frontiers in plant science · 2022Article
- Chlamydomonas as a model for reactive oxygen species signaling and thiol redox regulation in the green lineage.Plant physiology · 2021Review
- A theoretical framework of the hybrid mechanism of photosystem II photodamage.Photosynthesis research · 2021Article
- Elevating Ascorbate in Arabidopsis Stimulates the Production of Abscisic Acid, Phaseic Acid, and to a Lesser Extent Auxin (IAA) and Jasmonates, Resulting in Increased Expression ofInternational journal of molecular sciences · 2021Article
- The highly efficient NDH-dependent photosystem I cyclic electron flow pathway in the marine angiosperm Zostera marina.Photosynthesis research · 2020Article
- Ascorbate and Thiamin: Metabolic Modulators in Plant Acclimation Responses.Plants (Basel, Switzerland) · 2020Review
- Ascorbate Deficiency Does Not Limit Nonphotochemical Quenching inPlant physiology · 2020Article
- Article
- Sexual ReproductionFrontiers in plant science · 2020Article
- A guide to Open-JIP, a low-cost open-source chlorophyll fluorometer.Photosynthesis research · 2019Article
- Ascorbic acid metabolism and functions: A comparison of plants and mammals.Free radical biology & medicine · 2018Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously, we showed that in barley (Hordeum vulgare) leaves with heat-inactivated oxygen-evolving complexes, photosystem II (PSII) has access to a large pool of alternative electron donors. Based on in vitro data, we proposed that this donor was ascorbate, yet this hypothesis has not been substantiated in vivo. In this paper, with the aid of chlorophyll a fluorescence induced by short (5-ms) light pulses and 820-nm absorbance transient measurements on wild-type and ascorbate-deficient (vtc2-1) mutant leaves of Arabidopsis (Arabidopsis thaliana), we show that in heat-treated leaves the rate of electron donation to PSII as well as the 3-(3,4-dichlorophenyl)-1,1-dimethylurea-sensitive electron transport toward photosystem I depend on the ascorbate content of the leaves: upon ascorbate treatment, the donation half-time in the wild type and the mutant decreased from 25 to 22 ms and from 55 to 32 ms, respectively. Thermoluminescence measurements show that Tyr(Z)(+) is involved in the electron transfer from ascorbate to PSII. These data and the similar ascorbate dependencies of the heat-treated and the tris(hydroxymethyl)aminomethane-treated thylakoid membranes, with maximal donation half-times of about 16 ms, show that ascorbate is capable of supporting a sustained electron transport activity in leaves containing inactivated oxygen-evolving complexes. This alternative electron transport appears to be ubiquitous in the plant kingdom and is present in the green alga Chlamydomonas reinhardtii, and its rate depends on the physiological state of the plants and on environmental conditions. Our data suggest that ascorbate, as an alternative PSII electron donor, plays a physiological role in heat-stressed plants.
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