ArticleAmerican journal of physiology. Regulatory, integrative and comparative physiology2009
Functional characterization of Actinobacillus pleuropneumoniae urea transport protein, ApUT.
Article in American journal of physiology. Regulatory, integrative and comparative physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 10 citations in OpenAlex.
- Protein Structures of Urea Transporters.Sub-cellular biochemistry · 2025Review
- Solvent accessible surface area-assessed molecular basis of osmolyte-induced protein stability.RSC advances · 2024Article
- Urea-aromatic interactions in biology.Biophysical reviews · 2020Review
- Modeling of flux, binding and substitution of urea molecules in the urea transporter dvUT.Journal of molecular graphics & modelling · 2017Article
- Structure of urea transporters.Sub-cellular biochemistry · 2014Review
- Energetic and molecular water permeation mechanisms of the human red blood cell urea transporter B.PloS one · 2013Article
- Mechanisms of molecular transport through the urea channel of Helicobacter pylori.Nature communications · 2013Article
- Transport kinetics and selectivity of HpUreI, the urea channel from Helicobacter pylori.Biochemistry · 2011Article
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Authors and funding
5 authors at 1 institution in 1 country.
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Abstract
Urea transporters (UTs) effect rapid flux of urea across biological membranes. In the mammalian kidney, UT activity is essential for effective urine concentration. In bacteria, UT-mediated urea uptake permits intracellular urease to degrade urea to ammonia and CO(2), a process that either buffers acid loads or provides nutrient nitrogen. We have characterized the urea transport channel protein ApUT from Actinobacillus pleuropneumoniae. Kinetic analysis of bacterial inside-out membranes enriched in ApUT showed approximately 28-fold increase in urea permeability (3.3 +/- 0.4 x 10(-4) cm/s) compared with control vesicles (0.11 +/- 0.02 x 10(-4) cm/s). In addition to urea, ApUT also conducts water. Urea and water transport across the channel was phloretin and mercury inhibitable, and the site of inhibition may be located on the cytoplasmic side of the protein. Glycerol and urea analogs, such as methylamine, dimethylurea, formamide, acetamide, methylurea, propanamide, and ethylamine did not permeate across ApUT.
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