ArticleThe Journal of biological chemistry2009
beta1 integrin cytoplasmic domain residues selectively modulate fibronectin matrix assembly and cell spreading through talin and Akt-1.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Article
- Tensin3 interaction with talin drives the formation of fibronectin-associated fibrillar adhesions.The Journal of cell biology · 2022Article
- Cell-derived decellularized extracellular matrices.Methods in cell biology · 2018Article
- Importance of Interaction between Integrin and Actin Cytoskeleton in Suspension Adaptation of CHO cells.Applied biochemistry and biotechnology · 2016Article
- The interaction of Gα13 with integrin β1 mediates cell migration by dynamic regulation of RhoA.Molecular biology of the cell · 2015Article
- Contributions of the integrin β1 tail to cell adhesive forces.Experimental cell research · 2015Article
- The G protein-coupled estrogen receptor-1, GPER-1, promotes fibrillogenesis via a Shc-dependent pathway resulting in anchorage-independent growth.Hormones & cancer · 2014Article
- Talin-bound NPLY motif recruits integrin-signaling adapters to regulate cell spreading and mechanosensing.The Journal of cell biology · 2014Article
- Mechanisms of talin-dependent integrin signaling and crosstalk.Biochimica et biophysica acta · 2014Review
- Domain 2 of uPAR regulates single-chain urokinase-mediated angiogenesis through β1-integrin and VEGFR2.American journal of physiology. Heart and circulatory physiology · 2013Article
- Trop-2 inhibits prostate cancer cell adhesion to fibronectin through the β1 integrin-RACK1 axis.Journal of cellular physiology · 2012Article
- Full activity of the deleted in liver cancer 1 (DLC1) tumor suppressor depends on an LD-like motif that binds talin and focal adhesion kinase (FAK).Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Plasma and cellular fibronectin: distinct and independent functions during tissue repair.Fibrogenesis & tissue repair · 2011Article
- Osteoblast mineralization requires beta1 integrin/ICAP-1-dependent fibronectin deposition.The Journal of cell biology · 2011Article
- Article
- Effects of transfection of ICAP-1α and its mutants on adhesion and migration of 2H-11 cells.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2010Article
- Article
- Factor XII stimulates ERK1/2 and Akt through uPAR, integrins, and the EGFR to initiate angiogenesis.Blood · 2010Article
- Tensin 2 modulates cell contractility in 3D collagen gels through the RhoGAP DLC1.Journal of cellular biochemistry · 2010Article
- Assembly of fibronectin extracellular matrix.Annual review of cell and developmental biology · 2010Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
The integrin beta(1) cytoplasmic domain (tail) serves as a scaffold for numerous intracellular proteins. The mechanisms by which the tail coordinates these proteins to facilitate extracellular matrix assembly and cell spreading are not clear. This study demonstrates that the beta(1) cytoplasmic domain can regulate cell spreading on fibronectin and fibronectin matrix assembly through Akt- and talin-dependent mechanisms, respectively. To identify these mechanisms, we characterized GD25 cells expressing the beta(1) integrin cytoplasmic domain mutants W775A and R760A. Although cell spreading appears normal in R760A mutant-integrin cells compared with wild type, it is inhibited in W775A mutant cells. In contrast, both mutant cell lines show defective fibronectin matrix assembly. Inhibition of cell spreading, but not matrix assembly, in the W775A mutant cells is due to a specific defect in Akt-1 activation. In addition, we find that both W775A and R760A mutant integrins have reduced surface expression of the 9EG7 epitope that correlates with reduced recruitment of talin to beta(1) integrin cytoplasmic complexes. Down-regulation of talin with small interfering RNA or expression of green fluorescent protein-talin head domain inhibits matrix assembly in beta(1) wild-type cells, mimicking the defect seen with the W775A and R760A mutant cells. These results demonstrate distinct mechanisms by which integrins regulate cell spreading and matrix assembly through the beta(1) integrin cytoplasmic tail.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.