ArticleDevelopment (Cambridge, England)2009
no poles encodes a predicted E3 ubiquitin ligase required for early embryonic development of Drosophila.
Article in Development (Cambridge, England), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Bendless-mediated K63 ubiquitination modulates cellular signalling to regulate Drosophila hematopoiesis.Cell communication and signaling : CCS · 2026Article
- The PP2A-B56 Binding Site LxxIxE Contributes to Asp-Mediated Spindle Pole Stability.Cytoskeleton (Hoboken, N.J.) · 2025Article
- Silencing TRAIP suppresses cell proliferation and migration/invasion of triple negative breast cancer via RB-E2F signaling and EMT.Cancer gene therapy · 2023Article
- RNA-binding FMRP and Staufen sequentially regulate the Coracle scaffold to control synaptic glutamate receptor and bouton development.Development (Cambridge, England) · 2022Article
- Traip controls mushroom body size by suppressing mitotic defects.Development (Cambridge, England) · 2022Article
- Translesion DNA synthesis-driven mutagenesis in very early embryogenesis of fast cleaving embryos.Nucleic acids research · 2022Article
- TRAIP drives replisome disassembly and mitotic DNA repair synthesis at sites of incomplete DNA replication.eLife · 2019Article
- Nucleolar residence of the seckel syndrome protein TRAIP is coupled to ribosomal DNA transcription.Nucleic acids research · 2018Article
- Dual Regulation of Host TRAIP Post-translation and Nuclear/Plasma Distribution by Porcine Reproductive and Respiratory Syndrome Virus Non-structural Protein 1α Promotes Viral Proliferation.Frontiers in immunology · 2018Article
- TRAIP is involved in chromosome alignment and SAC regulation in mouse oocyte meiosis.Scientific reports · 2016Article
- TRAIP/RNF206 is required for recruitment of RAP80 to sites of DNA damage.Nature communications · 2016Article
- TRAIP regulates replication fork recovery and progression via PCNA.Cell discovery · 2016Article
- The TRAF-interacting protein (TRAIP) is a novel E2F target with peak expression in mitosis.Oncotarget · 2015Article
- The FHA domain determines Drosophila Chk2/Mnk localization to key mitotic structures and is essential for early embryonic DNA damage responses.Molecular biology of the cell · 2015Article
- Regulation of translesion DNA synthesis: Posttranslational modification of lysine residues in key proteins.DNA repair · 2015Review
- Tumor necrosis factor (TNF) receptor-associated factor (TRAF)-interacting protein (TRIP) negatively regulates the TRAF2 ubiquitin-dependent pathway by suppressing the TRAF2-sphingosine 1-phosphate (S1P) interaction.The Journal of biological chemistry · 2015Article
- Bendless modulates JNK-mediated cell death and migration in Drosophila.Cell death and differentiation · 2014Article
- TRIP/NOPO E3 ubiquitin ligase promotes ubiquitylation of DNA polymerase η.Development (Cambridge, England) · 2014Article
- Genetic screen for regulators of lymph gland homeostasis and hemocyte maturation in Drosophila.G3 (Bethesda, Md.) · 2012Article
- NOPO modulates Egr-induced JNK-independent cell death in Drosophila.Cell research · 2012Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
In a screen for cell-cycle regulators, we identified a Drosophila maternal effect-lethal mutant that we named ;no poles' (nopo). Embryos from nopo females undergo mitotic arrest with barrel-shaped, acentrosomal spindles during the rapid S-M cycles of syncytial embryogenesis. We identified CG5140, which encodes a candidate RING domain-containing E3 ubiquitin ligase, as the nopo gene. A conserved residue in the RING domain is altered in our EMS-mutagenized allele of nopo, suggesting that E3 ligase activity is crucial for NOPO function. We show that mutation of a DNA checkpoint kinase, CHK2, suppresses the spindle and developmental defects of nopo-derived embryos, revealing that activation of a DNA checkpoint operational in early embryos contributes significantly to the nopo phenotype. CHK2-mediated mitotic arrest has been previously shown to occur in response to mitotic entry with DNA damage or incompletely replicated DNA. Syncytial embryos lacking NOPO exhibit a shorter interphase during cycle 11, suggesting that they may enter mitosis prior to the completion of DNA replication. We show that Bendless (BEN), an E2 ubiquitin-conjugating enzyme, interacts with NOPO in a yeast two-hybrid assay; furthermore, ben-derived embryos arrest with a nopo-like phenotype during syncytial divisions. These data support our model that an E2-E3 ubiquitination complex consisting of BEN-UEV1A (E2 heterodimer) and NOPO (E3 ligase) is required for the preservation of genomic integrity during early embryogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.