ArticleDevelopment (Cambridge, England)2009
An in vitro ES cell imprinting model shows that imprinted expression of the Igf2r gene arises from an allele-specific expression bias.
Article in Development (Cambridge, England), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 60 citations in OpenAlex.
- Why are imprints unstable in pluripotent stem cells?Biochemical Society transactions · 2025Article
- Isogenic comparison of Airn and Xist reveals core principles of Polycomb recruitment by lncRNAs.Molecular cell · 2025Article
- Imprinted Long Non-Coding RNAs in Mammalian Development and Disease.International journal of molecular sciences · 2023Review
- Exploring chromatin structural roles of non-coding RNAs at imprinted domains.Biochemical Society transactions · 2021Review
- Evolution of Genome-Organizing Long Non-coding RNAs in Metazoans.Frontiers in genetics · 2020Review
- A human somatic cell culture system for modelling gene silencing by transcriptional interference.Heliyon · 2020Article
- Maternal transmission of an Igf2r domain 11: IGF2 binding mutant allele (Igf2rScientific reports · 2019Article
- A piggyBac-based toolkit for inducible genome editing in mammalian cells.RNA (New York, N.Y.) · 2019Article
- Allele-specific RNA-seq expression profiling of imprinted genes in mouse isogenic pluripotent states.Epigenetics & chromatin · 2019Article
- When Long Noncoding RNAs Meet Genome Editing in Pluripotent Stem Cells.Stem cells international · 2017Review
- Incorporating parent-of-origin effects in whole-genome prediction of complex traits.Genetics, selection, evolution : GSE · 2016Article
- Differential regulation of genomic imprinting by TET proteins in embryonic stem cells.Stem cell research · 2015Article
- Imprinted expression in cystic embryoid bodies shows an embryonic and not an extra-embryonic pattern.Developmental biology · 2015Article
- Article
- Genomic imprinting effects on complex traits in domesticated animal species.Frontiers in genetics · 2015Review
- Tissue-specific regulation of Igf2r/Airn imprinting during gastrulation.Epigenetics & chromatin · 2015Article
- H2A.B facilitates transcription elongation at methylated CpG loci.Genome research · 2014Article
- Genomic imprinting in mammals.Cold Spring Harbor perspectives in biology · 2014Review
- Expression of a large LINE-1-driven antisense RNA is linked to epigenetic silencing of the metastasis suppressor gene TFPI-2 in cancer.Nucleic acids research · 2013Article
- Gene regulation by the act of long non-coding RNA transcription.BMC biology · 2013Review
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
Genomic imprinting is an epigenetic process that results in parental-specific gene expression. Advances in understanding the mechanism that regulates imprinted gene expression in mammals have largely depended on generating targeted manipulations in embryonic stem (ES) cells that are analysed in vivo in mice. However, genomic imprinting consists of distinct developmental steps, some of which occur in post-implantation embryos, indicating that they could be studied in vitro in ES cells. The mouse Igf2r gene shows imprinted expression only in post-implantation stages, when repression of the paternal allele has been shown to require cis-expression of the Airn non-coding (nc) RNA and to correlate with gain of DNA methylation and repressive histone modifications. Here we follow the gain of imprinted expression of Igf2r during in vitro ES cell differentiation and show that it coincides with the onset of paternal-specific expression of the Airn ncRNA. Notably, although Airn ncRNA expression leads, as predicted, to gain of repressive epigenetic marks on the paternal Igf2r promoter, we unexpectedly find that the paternal Igf2r promoter is expressed at similar low levels throughout ES cell differentiation. Our results further show that the maternal and paternal Igf2r promoters are expressed equally in undifferentiated ES cells, but during differentiation expression of the maternal Igf2r promoter increases up to 10-fold, while expression from the paternal Igf2r promoter remains constant. This indicates, contrary to expectation, that the Airn ncRNA induces imprinted Igf2r expression not by silencing the paternal Igf2r promoter, but by generating an expression bias between the two parental alleles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.