ReviewMolecular pharmacology2009
Fluorescent and bioluminescent protein-fragment complementation assays in the study of G protein-coupled receptor oligomerization and signaling.
Review in Molecular pharmacology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 46 citations in OpenAlex.
- Oligomerization of transmembrane adenylyl cyclase isoforms.Molecular pharmacology · 2026Review
- Arrestin recruitment and signaling by G protein-coupled receptor heteromers.Neuropharmacology · 2019Review
- Luminescence- and Fluorescence-Based Complementation Assays to Screen for GPCR Oligomerization: Current State of the Art.International journal of molecular sciences · 2019Review
- Split Green Fluorescent Proteins: Scope, Limitations, and Outlook.Annual review of biophysics · 2019Review
- Surveying the landscape of optogenetic methods for detection of protein-protein interactions.Wiley interdisciplinary reviews. Systems biology and medicine · 2018Review
- Fungal G-protein-coupled receptors: mediators of pathogenesis and targets for disease control.Nature microbiology · 2018Review
- Methods used to study the oligomeric structure of G-protein-coupled receptors.Bioscience reports · 2017Review
- Dual-Color Luciferase Complementation for Chemokine Receptor Signaling.Methods in enzymology · 2016Article
- Propensity scores for prediction and characterization of bioluminescent proteins from sequences.PloS one · 2014Article
- Protein fragment bimolecular fluorescence complementation analyses for the in vivo study of protein-protein interactions and cellular protein complex localizations.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Novel split-luciferase-based genetically encoded biosensors for noninvasive visualization of Rho GTPases.PloS one · 2013Article
- Prediction of bioluminescent proteins using auto covariance transformation of evolutional profiles.International journal of molecular sciences · 2012Article
- Fluorescent protein complementation assays: new tools to study G protein-coupled receptor oligomerization and GPCR-mediated signaling.Molecular and cellular endocrinology · 2011Review
- Local clustering of transferrin receptors promotes clathrin-coated pit initiation.The Journal of cell biology · 2010Article
- Oligomeric forms of G protein-coupled receptors (GPCRs).Trends in biochemical sciences · 2010Article
- Visualization of G protein-coupled receptor (GPCR) interactions in living cells using bimolecular fluorescence complementation (BiFC).Current protocols in neuroscience · 2010Article
- Ligand-induced regulation and localization of cannabinoid CB1 and dopamine D2L receptor heterodimers.The Journal of pharmacology and experimental therapeutics · 2010Article
- Bimolecular fluorescence complementation: lighting up seven transmembrane domain receptor signalling networks.British journal of pharmacology · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Most cellular functions, including signaling by G protein-coupled receptors (GPCRs), are mediated by protein-protein interactions, making the identification and localization of protein complexes key to the understanding of cellular processes. In complement to traditional biochemical techniques, noninvasive resonance energy transfer (RET) and protein-fragment complementation assays (PCAs) now allow protein interactions to be detected in the context of living cells. In this review, fluorescent and bioluminescent PCAs are discussed and their application illustrated with studies on GPCR signaling. Newly developed techniques combining PCA and RET assays for the detection of ternary and quaternary protein complexes are also presented.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.