ArticleProceedings of the National Academy of Sciences of the United States of America2009
PolV(PolIVb) function in RNA-directed DNA methylation requires the conserved active site and an additional plant-specific subunit.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 79 citations in OpenAlex.
- Molecular Mechanisms Underlying the Establishment, Maintenance, and Removal of DNA Methylation in Plants.Annual review of plant biology · 2025Review
- CLSY docking to Pol IV requires a conserved domain critical for small RNA biogenesis and transposon silencing.Nature communications · 2024Article
- DNA-dependent RNA polymerases in plants.The Plant cell · 2023Article
- Pol V produced RNA facilitates transposable element excision site repair inmicroPublication biology · 2023Article
- Broad noncoding transcription suggests genome surveillance by RNA polymerase V.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Long-Read cDNA Sequencing Enables a "Gene-Like" Transcript Annotation of Transposable Elements.The Plant cell · 2020Article
- Involvement of MEM1 in DNA demethylation in Arabidopsis.Plant molecular biology · 2020Article
- Genome-Wide Reinforcement of DNA Methylation Occurs during Somatic Embryogenesis in Soybean.The Plant cell · 2019Article
- The NRPD1 N-terminus contains a Pol IV-specific motif that is critical for genome surveillance in Arabidopsis.Nucleic acids research · 2019Article
- Extensive profiling inRNA (New York, N.Y.) · 2019Article
- The RNA Export Factor ALY1 Enables Genome-Wide RNA-Directed DNA Methylation.The Plant cell · 2019Article
- Evidence for a Unique DNA-Dependent RNA Polymerase in Cereal Crops.Molecular biology and evolution · 2018Article
- RNA polymerases IV and V influence the 3' boundaries of Polymerase II transcription units in Arabidopsis.RNA biology · 2018Article
- The RNAs of RNA-directed DNA methylation.Biochimica et biophysica acta. Gene regulatory mechanisms · 2017Review
- The Argonaute-binding platform of NRPE1 evolves through modulation of intrinsically disordered repeats.The New phytologist · 2016Article
- Evidence for ARGONAUTE4-DNA interactions in RNA-directed DNA methylation in plants.Genes & development · 2016Article
- Long non-coding RNA produced by RNA polymerase V determines boundaries of heterochromatin.eLife · 2016Article
- Article
- RNA Pol IV and V in gene silencing: Rebel polymerases evolving away from Pol II's rules.Current opinion in plant biology · 2015Review
- Ancient Origin and Recent Innovations of RNA Polymerase IV and V.Molecular biology and evolution · 2015Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two forms of a plant-specific RNA polymerase (Pol), PolIV(PolIVa) and PolV(PolIVb), currently defined by their respective largest subunits [NRPD1(NRPD1a) and NRPE1(NRPD1b)], have been implicated in the production and activity of 24-nt small RNAs (sRNAs) in RNA-directed DNA methylation (RdDM). Prevailing models support the view that PolIV(PolIVa) plays an upstream role in RdDM by producing the 24-nt sRNAs, whereas PolV(PolIVb) would act downstream at a structural rather than an enzymatic level to reinforce sRNA production by PolIV(PolIVa) and mediate DNA methylation. However, the composition and mechanism of action of PolIV(PolIVa)/PolV(PolIVb) remain unclear. In this work, we have identified a plant-specific PolV(PolIVb) subunit, NRPE5a, homologous to NRPB5a, a common subunit shared by PolI-III and shown here to be present in PolIV(PolIVa). Our results confirm the combinatorial diversity of PolIV(PolIVa)/PolV(PolIVb) subunit composition and indicate that these plant-specific Pols are eukaryotic-type polymerases. Moreover, we show that nrpe5a-1 mutation differentially impacts sRNAs accumulation at various PolIV(PolIVa)/PolV(PolIVb)-dependent loci, indicating a target-specific requirement for NRPE5a in the process of PolV(PolIVb)-dependent gene silencing. We then describe that the triad aspartate motif present in the catalytic center of PolV(PolIVb) is required for recapitulation of all activities associated with this Pol complex in RdDM, suggesting that RNA polymerization is important for PolV(PolIVb) to perform its regulatory functions.
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