ArticleStructure (London, England : 1993)2009
The structure of phosphorylase kinase holoenzyme at 9.9 angstroms resolution and location of the catalytic subunit and the substrate glycogen phosphorylase.
Article in Structure (London, England : 1993), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Hepatic Glycogen Storage Disease Type IX: Long-Term Outcomes in the UK From 89 Patients.Journal of inherited metabolic disease · 2026Observational
- Liver-directed AAV gene therapy in mice corrects glycogen storage disease type IX γ2.Science advances · 2025Article
- Molecular basis for the regulation of human phosphorylase kinase by phosphorylation and CaNature communications · 2025Article
- Progressive liver disease and dysregulated glycogen metabolism in murine GSD IX γ2 models human disease.Molecular genetics and metabolism · 2024Article
- Architecture and activation of human muscle phosphorylase kinase.Nature communications · 2024Article
- Characterization of liver GSD IX γ2 pathophysiology in a novel Phkg2Molecular genetics and metabolism · 2021Article
- Chemically reprogramming the phospho-transfer reaction to crosslink protein kinases to their substrates.Protein science : a publication of the Protein Society · 2019Article
- Brain Glycogen Structure and Its Associated Proteins: Past, Present and Future.Advances in neurobiology · 2019Review
- The regulation of glycogenolysis in the brain.The Journal of biological chemistry · 2018Review
- Cryo-electron microscopy and X-ray crystallography: complementary approaches to structural biology and drug discovery.Acta crystallographica. Section F, Structural biology communications · 2017Review
- The regulatory α and β subunits of phosphorylase kinase directly interact with its substrate, glycogen phosphorylase.Biochemical and biophysical research communications · 2017Article
- Protein post-translational modifications:Computational and structural biotechnology journal · 2017Review
- Sensitive, nonradioactive assay of phosphorylase kinase through measurement of enhanced phosphorylase activity towards fluorogenic dextrin.Journal of biochemistry · 2016Article
- Mass Spectrometric Analysis of Surface-Exposed Regions in the Hexadecameric Phosphorylase Kinase Complex.Biochemistry · 2015Article
- Mass spectrometry reveals differences in stability and subunit interactions between activated and nonactivated conformers of the (αβγδ)4 phosphorylase kinase complex.Molecular & cellular proteomics : MCP · 2012Article
- Louise N. Johnson 1940-2012.Nature structural & molecular biology · 2012Article
- Structure and location of the regulatory β subunits in the (αβγδ)4 phosphorylase kinase complex.The Journal of biological chemistry · 2012Article
- Education catching up with science: preparing students for three-dimensional literacy in cell biology.CBE life sciences education · 2012Article
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
Phosphorylase kinase (PhK) coordinates hormonal and neuronal signals to initiate the breakdown of glycogen. The enzyme catalyzes the phosphorylation of inactive glycogen phosphorylase b (GPb), resulting in the formation of active glycogen phosphorylase a. We present a 9.9 angstroms resolution structure of PhK heterotetramer (alphabetagammadelta)4 determined by cryo-electron microscopy single-particle reconstruction. The enzyme has a butterfly-like shape comprising two lobes with 222 symmetry. This three-dimensional structure has allowed us to dock the catalytic gamma subunit to the PhK holoenzyme at a location that is toward the ends of the lobes. We have also determined the structure of PhK decorated with GPb at 18 angstroms resolution, which shows the location of the substrate near the kinase subunit. The PhK preparation contained a number of smaller particles whose structure at 9.8 angstroms resolution was consistent with a proteolysed activated form of PhK that had lost the alpha subunits and possibly the gamma subunits.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.