ArticleJournal of the American Chemical Society2009
Using NMR to distinguish viscosity effects from nonspecific protein binding under crowded conditions.
Article in Journal of the American Chemical Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The trial behind it
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Who cites it
28 citing papers in PubMed.
- GRAMMCell: Docking-based Cell Modeling Resource.Journal of molecular biology · 2025Article
- Diffusion and Viscosity in Mixed Protein Solutions.The journal of physical chemistry. B · 2024Article
- Optimized OPEP Force Field for Simulation of Crowded Protein Solutions.The journal of physical chemistry. B · 2023Article
- Preferential Interactions of a Crowder Protein with the Specific Binding Site of a Native Protein Complex.The journal of physical chemistry letters · 2022Article
- NMR Characterization of Surface Receptor Protein Interactions in Live Cells Using Methylcellulose Hydrogels.Angewandte Chemie (International ed. in English) · 2020Article
- Article
- The Inescapable Effects of Ribosomes on In-Cell NMR Spectroscopy and the Implications for Regulation of Biological Activity.International journal of molecular sciences · 2019Review
- Interaction proteomics by using in-cell NMR spectroscopy.Journal of proteomics · 2019Review
- Comparison of NMR and Dynamic Light Scattering for Measuring Diffusion Coefficients of Formulated Insulin: Implications for Particle Size Distribution Measurements in Drug Products.The AAPS journal · 2017Article
- Crowding in Cellular Environments at an Atomistic Level from Computer Simulations.The journal of physical chemistry. B · 2017Article
- Molecular Effects of Concentrated Solutes on Protein Hydration, Dynamics, and Electrostatics.Biophysical journal · 2016Article
- In-cell thermodynamics and a new role for protein surfaces.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Relative Cosolute Size Influences the Kinetics of Protein-Protein Interactions.Biophysical journal · 2015Article
- An osmolyte mitigates the destabilizing effect of protein crowding.Protein science : a publication of the Protein Society · 2014Article
- Impact of reconstituted cytosol on protein stability.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Ferritin: the protein nanocage and iron biomineral in health and in disease.Inorganic chemistry · 2013Review
- Amide proton exchange of a dynamic loop in cell extracts.Protein science : a publication of the Protein Society · 2013Article
- Protein dynamics elucidated by NMR technique.Protein & cell · 2013Article
- Effects of macromolecular crowding agents on protein folding in vitro and in silico.Biophysical reviews · 2013Review
- Soft interactions and crowding.Biophysical reviews · 2013Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Conventional NMR approaches to detect weak protein binding and aggregation are hindered by the increased viscosity brought about by crowding. We describe a simple and reliable NMR method to distinguish viscosity effects from binding and aggregation under crowded conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.