ArticleTransgenic research2009
Different subcellular localization and glycosylation for a functional antibody expressed in Nicotiana tabacum plants and suspension cells.
Article in Transgenic research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 70 citations in OpenAlex.
- Nicotiana hairy roots for recombinant protein expression, where to start? A systematic review.Molecular biology reports · 2023Pooled it
- Exploring recent progress of molecular farming for therapeutic and recombinant molecules in plant systems.Heliyon · 2024Review
- Production of Mature Recombinant Human Activin A in Transgenic Rice Cell Suspension Culture.Current issues in molecular biology · 2024Article
- In vivo deglycosylation of recombinant glycoproteins in tobacco BY-2 cells.Plant biotechnology journal · 2023Article
- Recent advances in expression and purification strategies for plant made vaccines.Frontiers in plant science · 2023Review
- Issue when expressing a recombinant protein under the control of pFrontiers in plant science · 2023Article
- Emerging trends of edible vaccine therapy for combating human diseases especially COVID-19: Pros, cons, and future challenges.Phytotherapy research : PTR · 2022Review
- Production of Recombinant Glycoproteins in Nicotiana tabacum BY-2 Suspension Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension Cells.International journal of molecular sciences · 2021Article
- Inactivation ofFrontiers in plant science · 2021Article
- Evolutionary and Predictive Functional Insights into the Aquaporin Gene Family in the Allotetraploid PlantInternational journal of molecular sciences · 2020Article
- Expression of Single Chain Variable Fragment (scFv) Molecules in Plants: A Comprehensive Update.Molecular biotechnology · 2020Review
- A review on edible vaccines and their prospects.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2020Review
- Overexpression of X Intrinsic Protein 1;1 inPlant direct · 2019Article
- The Nicotiana tabacum ABC transporter NtPDR3 secretes O-methylated coumarins in response to iron deficiency.Journal of experimental botany · 2018Article
- Activity of the purified plant ABC transporter NtPDR1 is stimulated by diterpenes and sesquiterpenes involved in constitutive and induced defenses.The Journal of biological chemistry · 2017Article
- Biomanufacturing of protective antibodies and other therapeutics in edible plant tissues for oral applications.Plant biotechnology journal · 2016Review
- An Accessory Protease Inhibitor to Increase the Yield and Quality of a Tumour-Targeting mAb in Nicotiana benthamiana Leaves.PloS one · 2016Article
- Tackling Unwanted Proteolysis in Plant Production Hosts Used for Molecular Farming.Frontiers in plant science · 2016Review
- Extracellular peptidase hunting for improvement of protein production in plant cells and roots.Frontiers in plant science · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genes encoding the heavy and light chains of LO-BM2, a therapeutic IgG antibody, were assembled in the tandem or inverted convergent orientation and expressed in Nicotiana tabacum plants and BY-2 suspension cells. The tandem construct allowed higher expression in both expression systems. A similar degradation pattern was observed for the secreted antibody recovered from the leaf intercellular fluid and BY-2 culture medium. Degradation increased with leaf age or culture time. Antibodies purified from leaf tissues and BY-2 cells were both functional. However, MS analysis of the N-glycosylation showed complex plant-type glycans to be the major type in the antibody purified from plants, whereas, oligomannosidic was the major glycosylation type in that purified from BY-2 cells. LO-BM2 was observed mainly in the endoplasmic reticulum of BY-2 cells while, in leaf cells, it was localized mostly to vesicles resembling prevacuolar compartments. These results and those from endoglycosidase H studies suggest that LO-BM2 is secreted from BY-2 cells more readily than from leaf cells where it accumulates in a post-Golgi compartment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.