ArticleProceedings of the National Academy of Sciences of the United States of America2009
Libraries against libraries for combinatorial selection of replicating antigen-antibody pairs.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Antibody Affinity Maturation by Yeast Mating against a Diverse Antigen Library Enhances Antibody Resistance to Viral Mutations.ACS pharmacology & translational science · 2026Article
- Library-on-Library Intercellular Labeling for Selection of Biotin Ligase and Acceptor Peptides.Chembiochem : a European journal of chemical biology · 2026Article
- Expanding the landscape of antibody discovery.Cell reports methods · 2024Article
- Article
- A library-on-library screen reveals the breadth expansion landscape of a broadly neutralizing betacoronavirus antibody.bioRxiv : the preprint server for biology · 2024Article
- Deploying synthetic coevolution and machine learning to engineer protein-protein interactions.Science (New York, N.Y.) · 2023Article
- Directed evolution of orthogonal RNA-RBP pairs through library-vs-library in vitro selection.Nucleic acids research · 2022Article
- High-resolution antibody dynamics of vaccine-induced immune responses.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Cells and cell lysates: a direct approach for engineering antibodies against membrane proteins using yeast surface display.Methods (San Diego, Calif.) · 2013Review
- Construction of a rationally designed antibody platform for sequencing-assisted selection.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Ribosome display of combinatorial antibody libraries derived from mice immunized with heat-killed Xylella fastidiosa and the selection of MopB-specific single-chain antibodies.Applied and environmental microbiology · 2012Article
- Mapping protein interactions by combining antibody affinity maturation and mass spectrometry.Analytical biochemistry · 2011Article
- Phenotype-information-phenotype cycle for deconvolution of combinatorial antibody libraries selected against complex systems.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Article
- Expanding the versatility of phage display I: efficient display of peptide-tags on protein VII of the filamentous phage.PloS one · 2011Article
- Internalizing cancer antibodies from phage libraries selected on tumor cells and yeast-displayed tumor antigens.Journal of molecular biology · 2010Article
- Interaction-dependent PCR: identification of ligand-target pairs from libraries of ligands and libraries of targets in a single solution-phase experiment.Journal of the American Chemical Society · 2010Article
- Combinatorial libraries against libraries for selecting neoepitope activation-specific antibodies.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- A novel strategy for proteome-wide ligand screening using cross-linked phage matrices.The Journal of biological chemistry · 2010Article
- Progress in phage display: evolution of the technique and its application.Cellular and molecular life sciences : CMLS · 2010Review
Corrections and comments
- Commented on by
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antibodies are among the most highly selective tight-binding ligands for proteins. Because the human genome project has deciphered the proteome, there is an opportunity to use combinatorial antibody libraries to select high-affinity antibodies to every protein encoded by the genome. However, this is a large task because the selection formats used today for combinatorial antibody libraries are geared toward generating antibodies to one antigen at a time. Here, we describe a method that accelerates the identification of antibodies to a multitude of antigens simultaneously by matching combinatorial antibody libraries against eukaryotic antigen libraries so that replication-competent cognate antigen-antibody pairs can be directly selected. Phage and yeast display systems are used because they each link genotype to phenotype and can be replicated individually. When combined with cell sorting, the two libraries can be selected against each other for recovery of cognate antigen-antibody clones in a single experiment.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.