ArticleThe Journal of cell biology2009
Golgi targeting of Drosophila melanogaster beta4GalNAcTB requires a DHHC protein family-related protein as a pilot.
Article in The Journal of cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Analysis of biochemical features of ST8 α-N-acetyl-neuraminide α2,8-sialyltransferase (St8sia) 5 isoforms.Glycoconjugate journal · 2022Article
- Article
- Genome-wide CRISPR screens for Shiga toxins and ricin reveal Golgi proteins critical for glycosylation.PLoS biology · 2018Article
- Distribution of Glycan Motifs at the Surface of Midgut Cells in the Cotton Leafworm (Frontiers in physiology · 2017Article
- Golgi UDP-GlcNAc:polypeptide O-α-N-Acetyl-d-glucosaminyltransferase 2 (TcOGNT2) regulates trypomastigote production and function in Trypanosoma cruzi.Eukaryotic cell · 2014Article
- Ion channel regulation by protein S-acylation.The Journal of general physiology · 2014Review
- Identification of a novel protein binding motif within the T-synthase for the molecular chaperone Cosmc.The Journal of biological chemistry · 2014Article
- The N's and O's of Drosophila glycoprotein glycobiology.Glycoconjugate journal · 2013Review
- Genetic Interactions Between Drosophila sialyltransferase and β1,4-N-acetylgalactosaminyltransferase-A Genes Indicate Their Involvement in the Same Pathway.G3 (Bethesda, Md.) · 2012Article
- Tight complex formation between Cosmc chaperone and its specific client non-native T-synthase leads to enzyme activity and client-driven dissociation.The Journal of biological chemistry · 2012Article
- "Add-on" domains of Drosophila β1,4-N-acetylgalactosaminyltransferase B in the stem region and its pilot protein.Cellular and molecular life sciences : CMLS · 2011Article
- Fc-glycosylation of IgG1 is modulated by B-cell stimuli.Molecular & cellular proteomics : MCP · 2011Article
- Localization of Golgi-resident glycosyltransferases.Cellular and molecular life sciences : CMLS · 2010Review
- Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: an update for 2009-2010.Mass spectrometry reviewsReview
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drosophila melanogaster beta4GalNAcTB mutant flies revealed that this particular N-acetylgalactosaminyltransferase is predominant in the formation of lacdiNAc (GalNAcbeta1,4GlcNAc)-modified glycolipids, but enzymatic activity could not be confirmed for the cloned enzyme. Using a heterologous expression cloning approach, we isolated beta4GalNAcTB together with beta4GalNAcTB pilot (GABPI), a multimembrane-spanning protein related to Asp-His-His-Cys (DHHC) proteins but lacking the DHHC consensus sequence. In the absence of GABPI, inactive beta4GalNAcTB is trapped in the endoplasmic reticulum (ER). Coexpression of beta4GalNAcTB and GABPI generates the active enzyme that is localized together with GABPI in the Golgi. GABPI associates with beta4GalNAcTB and, when expressed with an ER retention signal, holds active beta4GalNAcTB in the ER. Importantly, treatment of isolated membrane vesicles with Triton X-100 disturbs beta4GalNAcTB activity. This phenomenon occurs with multimembrane-spanning glycosyltransferases but is normally not a property of glycosyltransferases with one membrane anchor. In summary, our data provide evidence that GABPI is required for ER export and activity of beta4GalNAcTB.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.