ArticleThe Journal of cell biology2009
Sustained activation of STAT5 is essential for chromatin remodeling and maintenance of mammary-specific function.
Article in The Journal of cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
59 citing papers in PubMed, 0 citations in OpenAlex.
- A long-lasting prolactin stimulates galactopoiesis in mice.iScience · 2025Article
- A New Perspective on Precision Medicine: The Power of Digital Organoids.Biomaterials research · 2025Review
- New Design Rules for Developing Potent Cell-Active Inhibitors of the Nucleosome Remodeling Factor (NURF) via BPTF Bromodomain Inhibition.Journal of medicinal chemistry · 2021Article
- Tumor organoid models in precision medicine and investigating cancer-stromal interactions.Pharmacology & therapeutics · 2021Review
- Oncogenic D816V-KIT signaling in mast cells causes persistent IL-6 production.Haematologica · 2020Article
- In Vitro and Ex Vivo Models - The Tumor Microenvironment in a Flask.Advances in experimental medicine and biology · 2020Review
- Matrix stiffness induces a tumorigenic phenotype in mammary epithelium through changes in chromatin accessibility.Nature biomedical engineering · 2019Article
- Deep RNA-Seq reveals miRNome differences in mammary tissue of lactating Holstein and Montbéliarde cows.BMC genomics · 2019Article
- Constitutive STAT5 activation regulates Paneth and Paneth-like cells to controlLife science alliance · 2019Article
- The Physical and Biochemical Properties of the Extracellular Matrix Regulate Cell Fate.Current topics in developmental biology · 2018Review
- Zinc transporter 2 interacts with vacuolar ATPase and is required for polarization, vesicle acidification, and secretion in mammary epithelial cells.The Journal of biological chemistry · 2017Article
- Article
- Orientation and repositioning of chromosomes correlate with cell geometry-dependent gene expression.Molecular biology of the cell · 2017Article
- Nectin-4 co-stimulates the prolactin receptor by interacting with SOCS1 and inhibiting its activity on the JAK2-STAT5a signaling pathway.The Journal of biological chemistry · 2017Article
- Deep nuclear invaginations are linked to cytoskeletal filaments - integrated bioimaging of epithelial cells in 3D culture.Journal of cell science · 2017Article
- Increased ROS production in non-polarized mammary epithelial cells induces monocyte infiltration in 3D culture.Journal of cell science · 2017Article
- Nuclear lamins in cancer.Cellular and molecular bioengineering · 2016Article
- A Novel Nectin-mediated Cell Adhesion Apparatus That Is Implicated in Prolactin Receptor Signaling for Mammary Gland Development.The Journal of biological chemistry · 2016Article
- Development of an ex vivo breast cancer lung colonization model utilizing a decellularized lung matrix.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Physical, Spatial, and Molecular Aspects of Extracellular Matrix of In Vivo Niches and Artificial Scaffolds Relevant to Stem Cells Research.Stem cells international · 2015Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Epithelial cells, once dissociated and placed in two-dimensional (2D) cultures, rapidly lose tissue-specific functions. We showed previously that in addition to prolactin, signaling by laminin-111 was necessary to restore functional differentiation of mammary epithelia. Here, we elucidate two additional aspects of laminin-111 action. We show that in 2D cultures, the prolactin receptor is basolaterally localized and physically segregated from its apically placed ligand. Detachment of the cells exposes the receptor to ligation by prolactin leading to signal transducers and activators of transcription protein 5 (STAT5) activation, but only transiently and not sufficiently for induction of milk protein expression. We show that laminin-111 reorganizes mammary cells into polarized acini, allowing both the exposure of the prolactin receptor and sustained activation of STAT5. The use of constitutively active STAT5 constructs showed that the latter is necessary and sufficient for chromatin reorganization and beta-casein transcription. These results underscore the crucial role of continuous laminin signaling and polarized tissue architecture in maintenance of transcription factor activation, chromatin organization, and tissue-specific gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.