ArticleThe Journal of cell biology2009
The nuclear export factor Xpo1p targets Mad1p to kinetochores in yeast.
Article in The Journal of cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Multifunctionality of F-rich nucleoporins.Biochemical Society transactions · 2020Review
- Synthetic Physical Interactions Map Kinetochore-Checkpoint Activation Regions.G3 (Bethesda, Md.) · 2016Article
- Control of the spindle checkpoint by lateral kinetochore attachment and limited Mad1 recruitment.Molecular biology of the cell · 2015Article
- LocNES: a computational tool for locating classical NESs in CRM1 cargo proteins.Bioinformatics (Oxford, England) · 2015Article
- A putative N-terminal nuclear export sequence is sufficient for Mps1 nuclear exclusion during interphase.BMC cell biology · 2015Article
- A multifunctional, multi-pathway intracellular localization signal in Huntingtin.Communicative & integrative biology · 2013Article
- A Mad that wears two hats: Mad1's control of nuclear trafficking.Developmental cell · 2013Article
- The yeast nuclear pore complex and transport through it.Genetics · 2012Article
- Laterally attached kinetochores recruit the checkpoint protein Bub1, but satisfy the spindle checkpoint.Cell cycle (Georgetown, Tex.) · 2010Article
- Nuclear transport and the mitotic apparatus: an evolving relationship.Cellular and molecular life sciences : CMLS · 2010Review
- The nuclear pore complex: bridging nuclear transport and gene regulation.Nature reviews. Molecular cell biology · 2010Review
- Double duty for nuclear proteins--the price of more open forms of mitosis.Trends in genetics : TIG · 2009Review
- A role for transportin in deposition of TTP to cytoplasmic RNA granules and mRNA decay.Nucleic acids research · 2009Article
- Dual personality of Mad1: regulation of nuclear import by a spindle assembly checkpoint protein.Nucleus (Austin, Tex.)Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Nuclear pore complexes (NPCs) mediate all nucleocytoplasmic traffic and provide docking sites for the spindle assembly checkpoint (SAC) protein Mad1p. Upon SAC activation, Mad1p is recruited onto kinetochores and rapidly cycles between NPCs and kinetochores. We examined the mechanism of Mad1p movement onto kinetochores and show that it is controlled by two components of the nuclear transport machinery, the exportin Xpo1p and Ran-guanosine triphosphate (GTP). Mad1p contains a nuclear export signal (NES) that is recognized by Xpo1p. The NES, Xpo1p, and RanGTP are all required for Mad1p recruitment onto kinetochores in checkpoint-activated cells. Consistent with this function, Xpo1p also accumulates on kinetochores after SAC activation. We have also shown that Xpo1p and RanGTP are required for the dynamic cycling of Mad1p between NPCs and kinetochores in checkpoint-arrested cells. These results reveal an important function for Xpo1p in mediating intranuclear transport events and identify a signaling pathway between kinetochores and NPCs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.