ArticleApplied and environmental microbiology2009
Comparison of loop-mediated isothermal amplification assay and conventional culture methods for detection of Campylobacter jejuni and Campylobacter coli in naturally contaminated chicken meat samples.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 76 citations in OpenAlex.
- Development of a novel rapid genome exponential amplification reaction (GEAR) assay for high-sensitivity detection of Campylobacter coli using simplified sample preparation for on-site detection.World journal of microbiology & biotechnology · 2026Article
- Comparative evaluation of 14Frontiers in microbiology · 2026Article
- Development and evaluation of loop-mediated isothermal amplification (lamp) for rapid detection of campylobacter jejuni.Revista peruana de medicina experimental y salud publica · 2025Article
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- A Cutoff Determination of Real-Time Loop-Mediated Isothermal Amplification (LAMP) for End-Point Detection ofVeterinary sciences · 2022Article
- Editorial:Frontiers in microbiology · 2022Article
- Detection ofBioMed research international · 2022Article
- Rapid detection of Clostridium perfringens in food by loop-mediated isothermal amplification combined with a lateral flow biosensor.PloS one · 2021Article
- Article
- Combined Loop-Mediated Isothermal Amplification Assays for Rapid Detection and One-Step Differentiation ofFrontiers in microbiology · 2021Article
- Loop-Mediated Isothermal Amplification (LAMP) as a Rapid, Affordable and Effective Tool to Involve Students in Undergraduate Research.Frontiers in microbiology · 2020Article
- Metagenomic analysis of isolation methods of a targeted microbe, Campylobacter jejuni, from chicken feces with high microbial contamination.Microbiome · 2019Article
- Classification of Multiple DNA Dyes Based on Inhibition Effects on Real-Time Loop-Mediated Isothermal Amplification (LAMP): Prospect for Point of Care Setting.Frontiers in microbiology · 2019Article
- A Sensitive, Specific and Simple Loop Mediated Isothermal Amplification Method for Rapid Detection ofFrontiers in microbiology · 2019Article
- Integrated LAMP and immunoassay platform for diarrheal disease detection.Biosensors & bioelectronics · 2018Article
- Generation and characterization of a novel recombinant scFv antibody specific for Campylobacter jejuni.Applied microbiology and biotechnology · 2018Article
- Molecular Tools To Study Preharvest Food Safety Challenges.Microbiology spectrum · 2018Review
- A Rapid LAMP-Based Method for Screening Poultry Samples forFrontiers in microbiology · 2018Article
- Use of Direct LAMP Screening of Broiler Fecal Samples forFrontiers in microbiology · 2016Article
- Comparison of conventional PCR, multiplex PCR, and loop-mediated isothermal amplification assays for rapid detection of Arcobacter species.Journal of clinical microbiology · 2014Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the efficacy of a loop-mediated isothermal amplification (LAMP) assay for detection of chicken meat samples naturally contaminated with Campylobacter jejuni and Campylobacter coli. A total of 144 Preston enrichment broth cultures from chicken meat samples were assessed by using the LAMP assay and conventional culture methods, which consist of a combination of Preston enrichment culturing and plating onto Butzler and modified charcoal cefoperazone deoxycholate agars. Compared with C. jejuni-C. coli isolation using the conventional culture test, the LAMP results showed 98.5% (67/68) and 97.4% (74/76) sensitivity and specificity, respectively, and the positive and negative predictive values were 97.1% (67/69) and 98.7% (74/75), respectively. The conventional culture test required more than 3 to 4 days to isolate and identify C. jejuni and C. coli in the Preston enrichment cultures. In contrast, the LAMP assay was markedly faster, requiring less than 90 min from the beginning of DNA extraction to final detection and differentiation of C. jejuni and C. coli. In total, the LAMP assay required 23.5 to 25.5 h from the beginning of the enrichment culture to final determination. These results suggest that our LAMP assay is a powerful tool for rapid, sensitive, and practical detection of C. jejuni and C. coli which may facilitate surveillance and control of C. jejuni-C. coli contamination in chicken, as well as investigations of food poisoning incidents caused by these organisms. This is the first report of a highly sensitive and specific LAMP assay to detect and differentiate C. jejuni and C. coli in chicken meat samples.
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Registered trials
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