Evidence map›Paper›PMID 19139238›Full record

ArticleApplied and environmental microbiology2009

Transcriptional regulation and signal-peptide-dependent secretion of exolevanase (LsdB) in the endophyte Gluconacetobacter diazotrophicus.

Carmen Menéndez, Alexander Banguela, Jesús Caballero-Mellado, Lázaro Hernández

Open access · greenAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Carmen MenéndezLaboratorio Interacciones Planta-Microorganismos, Centro de Ingeniería Genética y Biotecnología, Apartado 6162, Habana 10600, Cuba.
Alexander Banguela
Jesús Caballero-Mellado
Lázaro Hernández
Centro de Ingeniería Genética y Biotecnología · CUUniversidad Nacional Autónoma de México · MX

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gluconacetobacter diazotrophicus utilizes plant sucrose with a constitutively expressed levansucrase (LsdA), producing extracellular levan, which may be degraded under energetically unfavored conditions. Reverse transcriptase-PCR analysis revealed that lsdA and the downstream exolevanase gene (lsdB) form an operon. lsdB transcription was induced during growth with low fructose concentrations (0.44 to 33 mM) and repressed by glucose. Transport of LsdB to the periplasm involved N-terminal signal peptide cleavage. Type II secretion mutants failed to transfer LsdB across the outer membrane, impeding levan hydrolysis.

Indexed as

Gene Expression Regulation, BacterialTranscription, GeneticAmino Acid SequenceBacterial ProteinsBase SequenceFructansFructoseGene OrderGluconacetobacterGlucoseGlycoside HydrolasesMolecular Sequence DataOperonPeriplasmProtein Sorting SignalsReverse Transcriptase Polymerase Chain ReactionBacterial ProteinsFructansFructoseGlucoseGlycoside HydrolaseslevanaseProtein Sorting Signals

Identifiers

PMID19139238
PMCPMC2655460
OpenAlexW2086001650

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.