ArticleApplied and environmental microbiology2009
Sequencing and analysis of fungal rRNA operons for development of broad-range fungal PCR assays.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
55 citing papers in PubMed, 1 synthesis or guideline pooled it, 118 citations in OpenAlex.
- Culture-negative periprosthetic joint infection: prevalence, aetiology, evaluation, recommendations, and treatment.International orthopaedics · 2020Pooled it
- Effect of intrapartum azithromycin on early childhood gut mycobiota development: post hoc analysis of a double-blind randomized trial.Nature communications · 2025Trial
- Lean and Green Hand Surgery: Evaluating the Sterility of Stored Corticosteroid Injection Preparations Using Next-Generation Sequencing.Hand (New York, N.Y.) · 2026Article
- Fungi enhance microbial carbon retention in high Arctic fjord sediment.PLoS biology · 2026Article
- Case report: intracranial fungal granuloma in a dromedary (Camelus dromedarius).BMC veterinary research · 2026Article
- Molecular diagnostics in cancer patients with suspected respiratory mold infections.Journal of clinical microbiology · 2025Article
- Challenges and predictive value of morphologic features in panfungal sequencing of formalin-fixed, paraffin-embedded tissues: A 5-year retrospective study.American journal of clinical pathology · 2025Article
- Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections.Clinical microbiology reviews · 2024Review
- Development of a Multiplex Real-Time PCR Assay for the Simultaneous Detection of Two Fungal Pathogens Causing Pneumonia.Journal of fungi (Basel, Switzerland) · 2024Article
- ICU care through NGS - Based identification of infectious agents: A comparative study.Heliyon · 2024Article
- Powder diet exacerbates oropharyngeal candidiasis in a mouse model.Applied and environmental microbiology · 2024Article
- Ecological competition in the oral mycobiome of Hispanic adults living in Puerto Rico associates with periodontitis.Journal of oral microbiology · 2024Article
- Analysis of Mould Exposure of Immunosuppressed Patients at a German University Hospital.Microorganisms · 2023Article
- A prebiotic diet modulates the oral microbiome composition and results in the attenuation of oropharyngeal candidiasis in mice.Microbiology spectrum · 2023Article
- Molecular Identification of Candida albicans and C. dubliniensis Using Small Subunit rRNA Gene Sequence in Kerbala, Iraq.Archives of Razi Institute · 2023Article
- Screening of endophytic fungi from Cremastra appendiculata and their potential for plant growth promotion and biological control.Folia microbiologica · 2023Article
- Diagnosis of Aspergillosis in Horses.Journal of fungi (Basel, Switzerland) · 2023Article
- Structural and functional analysis of EntV reveals a 12 amino acid fragment protective against fungal infections.Nature communications · 2022Article
- A novel rRNA hybridization-based approach to rapid, accurate Candida identification directly from blood culture.Medical mycology · 2022Article
- Fungal periprosthetic joint infection: Rare but challenging problem.Chinese journal of traumatology = Zhonghua chuang shang za zhi · 2022Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
rRNA genes are attractive targets for developing PCR assays targeting human fungal pathogens. Most studies have focused on the 18S rRNA gene, internal transcribed spacers, and the 5' end of the 28S rRNA gene. An approximately 2,900-bp region of the 28S rRNA gene remains largely unexplored because sequences of many medically relevant fungi are either unavailable or undefined in genomic databases. The internal transcribed spacers and 28S rRNA gene of nine medically and phylogenetically important fungi were sequenced. In addition, 42 sequences from this region were acquired from public databases, resulting in an alignment of 51 fungal sequences spanning 30 fungal genera. For the nearly 3,950-bp region from the 3' end of 18S rRNA gene to the 3' end of the 28S rRNA gene, 27 broad-range PCR primers were designed such that their sequence homology with the human rRNA gene was minimal. All 62 possible amplicons in the size range from 75 to 400 bp from 27 primers were screened using fungal genomic DNA from 26 species spanning 14 genera. Eleven of the 62 amplicons did not cross-react with 1 microg/PCR human DNA but simultaneously amplified 10 fg of fungal DNA. Phylogenetic distance matrices were calculated for regions covered by these 11 amplicons based on 51 fungi. Two of these 11 amplicons successfully amplified 30 fg of fungal DNA from 25 of 26 fungi and provided the most phylogenetic information for species identification based on the distance matrices. These PCR assays hold promise for detection and identification of fungal pathogens in human tissues.
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