ArticleThe Journal of biological chemistry2009
Structure, binding, and activity of Syd, a SecY-interacting protein.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Capture of endogenous lipids in peptidiscs and effect on protein stability and activity.iScience · 2024Article
- Recent advances in membrane mimetics for membrane protein research.Biochemical Society transactions · 2023Review
- Uncovering the Optimal Molecular Characteristics of Hydrophobe-Containing Polypeptoids to Induce Liposome or Cell Membrane Fragmentation.Biomacromolecules · 2023Article
- The Dynamic SecYEG Translocon.Frontiers in molecular biosciences · 2021Review
- Investigating the stability of the SecA-SecYEG complex during protein translocation across the bacterial membrane.The Journal of biological chemistry · 2019Article
- Nanodiscs: A Controlled Bilayer Surface for the Study of Membrane Proteins.Annual review of biophysics · 2018Article
- Physicochemical factors controlling the activity and energy coupling of an ionic strength-gated ATP-binding cassette (ABC) transporter.The Journal of biological chemistry · 2013Article
- Nanodiscs as a new tool to examine lipid-protein interactions.Methods in molecular biology (Clifton, N.J.) · 2013Review
- Two copies of the SecY channel and acidic lipids are necessary to activate the SecA translocation ATPase.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Article
- Nanodisc-based co-immunoprecipitation for mass spectrometric identification of membrane-interacting proteins.Molecular & cellular proteomics : MCP · 2011Article
- A novel immunity system for bacterial nucleic acid degrading toxins and its recruitment in various eukaryotic and DNA viral systems.Nucleic acids research · 2011Article
- Non-vesicular transfer of membrane proteins from nanoparticles to lipid bilayers.The Journal of general physiology · 2011Article
- Lipid-protein correlations in nanoscale phospholipid bilayers determined by solid-state nuclear magnetic resonance.Biochemistry · 2010Article
- Membrane protein assembly into Nanodiscs.FEBS letters · 2010Review
- Chapter 11 - Reconstitution of membrane proteins in phospholipid bilayer nanodiscs.Methods in enzymology · 2009Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Syd protein has been implicated in the Sec-dependent transport of polypeptides across the bacterial inner membrane. Using Nanodiscs, we here provide direct evidence that Syd binds the SecY complex, and we demonstrate that interaction involves the two electropositive and cytosolic loops of the SecY subunit. We solve the crystal structure of Syd and together with cysteine cross-link analysis, we show that a conserved concave and electronegative groove constitutes the SecY-binding site. At the membrane, Syd decreases the activity of the translocon containing loosely associated SecY-SecE subunits, whereas in detergent solution Syd disrupts the SecYEG heterotrimeric associations. These results support the role of Syd in proofreading the SecY complex biogenesis and point to the electrostatic nature of the Sec channel interaction with its cytosolic partners.
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Registered trials
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