ArticleNucleic acids research2009
Steady-state levels of imported tRNAs in Chlamydomonas mitochondria are correlated with both cytosolic and mitochondrial codon usages.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 35 citations in OpenAlex.
- Assembly and Comparative Analysis of Five Complete Mitochondrial Genomes of theInternational journal of molecular sciences · 2026Article
- Extensive import of nucleus-encoded tRNAs into chloroplasts of the photosynthetic lycophyte,Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Rapid Shifts in Mitochondrial tRNA Import in a Plant Lineage with Extensive Mitochondrial tRNA Gene Loss.Molecular biology and evolution · 2021Article
- Mitochondrial Genome Evolution in Pelagophyte Algae.Genome biology and evolution · 2021Article
- The Functions of Chloroplast Glutamyl-tRNA in Translation and Tetrapyrrole Biosynthesis.Plant physiology · 2020Article
- tRNA biology in mitochondria.International journal of molecular sciences · 2015Review
- A plastid without a genome: evidence from the nonphotosynthetic green algal genus Polytomella.Plant physiology · 2014Article
- The mTERF protein MOC1 terminates mitochondrial DNA transcription in the unicellular green alga Chlamydomonas reinhardtii.Nucleic acids research · 2013Article
- The absence of A-to-I editing in the anticodon of plant cytoplasmic tRNA (Arg) ACG demands a relaxation of the wobble decoding rules.RNA biology · 2012Article
- Co-evolution of mitochondrial tRNA import and codon usage determines translational efficiency in the green alga Chlamydomonas.PLoS genetics · 2012Article
- Evolution of macromolecular import pathways in mitochondria, hydrogenosomes and mitosomes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2010Review
- Organization and expression of organellar genomes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2010Review
- Thiolated tRNAs of Trypanosoma brucei are imported into mitochondria and dethiolated after import.The Journal of biological chemistry · 2009Article
- Mitochondrial tRNA import--the challenge to understand has just begun.Biological chemistry · 2009Review
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mitochondrial genome of Chlamydomonas reinhardtii only encodes three expressed tRNA genes, thus most mitochondrial tRNAs are likely imported. The sharing of tRNAs between chloroplasts and mitochondria has been speculated in this organism. We first demonstrate that no plastidial tRNA is present in mitochondria and that the mitochondrial translation mainly relies on the import of nucleus-encoded tRNA species. Then, using northern analysis, we show that the extent of mitochondrial localization for the 49 tRNA isoacceptor families encoded by the C. reinhardtii nuclear genome is highly variable. Until now the reasons for such variability were unknown. By comparing cytosolic and mitochondrial codon usage with the sub-cellular distribution of tRNAs, we provide unprecedented evidence that the steady-state level of a mitochondrial tRNA is linked not only to the frequency of the cognate codon in mitochondria but also to its frequency in the cytosol, then allowing optimal mitochondrial translation.
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