ReviewThe New phytologist2008
Quantitative imaging for discovery and assembly of the metabo-regulome.
Review in The New phytologist, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Concept of Fluorescent Transport Activity Biosensor for the Characterization of the Arabidopsis NPF1.3 Activity of Nitrate.Sensors (Basel, Switzerland) · 2022Article
- Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology.Plant physiology · 2021Review
- A Fluorescence Resonance Energy Transfer-Based Analytical Tool for Nitrate Quantification in Living Cells.ACS omega · 2020Article
- Recent advances in imaging subcellular processes.F1000Research · 2016Review
- Single-cell imaging tools for brain energy metabolism: a review.Neurophotonics · 2014Article
- Article
- Visualization of plant cell wall lignification using fluorescence-tagged monolignols.The Plant journal : for cell and molecular biology · 2013Article
- Linking post-translational modifications and variation of phenotypic traits.Molecular & cellular proteomics : MCP · 2013Article
- New technologies for 21st century plant science.The Plant cell · 2012Article
- Optogenetic reporters: Fluorescent protein-based genetically encoded indicators of signaling and metabolism in the brain.Progress in brain research · 2012Review
- Quantitative glucose and ATP sensing in mammalian cells.Pharmaceutical research · 2011Review
- Optical sensors for measuring dynamic changes of cytosolic metabolite levels in yeast.Nature protocols · 2011Article
- Optical sensors for monitoring dynamic changes of intracellular metabolite levels in mammalian cells.Nature protocols · 2011Article
- Amino acid export in plants: a missing link in nitrogen cycling.Molecular plant · 2011Review
- Switch-based biosensors: a new approach towards real-time, in vivo molecular detection.Trends in biotechnology · 2011Article
- Engineering genetically encoded nanosensors for real-time in vivo measurements of citrate concentrations.PloS one · 2011Article
- Dynamic analysis of cytosolic glucose and ATP levels in yeast using optical sensors.The Biochemical journal · 2010Article
- H-independent glutamine transport in plant root tips.PloS one · 2010Article
- Genetically encoded biosensors based on engineered fluorescent proteins.Chemical Society reviews · 2009Review
- Comparison of quantitative metabolite imaging tools and carbon-13 techniques for fluxomics.Methods in molecular biology (Clifton, N.J.) · 2009Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Little is known about regulatory networks that control metabolic flux in plant cells. Detailed understanding of regulation is crucial for synthetic biology. The difficulty of measuring metabolites with cellular and subcellular precision is a major roadblock. New tools have been developed for monitoring extracellular, cytosolic, organellar and vacuolar ion and metabolite concentrations with a time resolution of milliseconds to hours. Genetically encoded sensors allow quantitative measurement of steady-state concentrations of ions, signaling molecules and metabolites and their respective changes over time. Fluorescence resonance energy transfer (FRET) sensors exploit conformational changes in polypeptides as a proxy for analyte concentrations. Subtle effects of analyte binding on the conformation of the recognition element are translated into a FRET change between two fused green fluorescent protein (GFP) variants, enabling simple monitoring of analyte concentrations using fluorimetry or fluorescence microscopy. Fluorimetry provides information averaged over cell populations, while microscopy detects differences between cells or populations of cells. The genetically encoded sensors can be targeted to subcellular compartments or the cell surface. Confocal microscopy ultimately permits observation of gradients or local differences within a compartment. The FRET assays can be adapted to high-throughput analysis to screen mutant populations in order to systematically identify signaling networks that control individual steps in metabolic flux.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.