ArticleJournal of the American Chemical Society2009
Long distance PELDOR measurements on the histone core particle.
Article in Journal of the American Chemical Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Chemical and biological tools for the preparation of modified histone proteins.Topics in current chemistry · 2015Article
- The spatial effect of protein deuteration on nitroxide spin-label relaxation: implications for EPR distance measurement.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2014Article
- Pulsed EPR distance measurements in soluble proteins by site-directed spin labeling (SDSL).Current protocols in protein science · 2013Article
- Optimization of pulsed DEER measurements for Gd-based labels: choice of operational frequencies, pulse durations and positions, and temperature.Applied magnetic resonance · 2013Article
- Improved Sensitivity for Long-Distance Measurements in Biomolecules: Five-Pulse Double Electron-Electron Resonance.The journal of physical chemistry letters · 2013Article
- Structural plasticity of histones H3-H4 facilitates their allosteric exchange between RbAp48 and ASF1.Nature structural & molecular biology · 2013Article
- Pulsed electron double resonance in structural studies of spin-labeled nucleic acids.Acta naturae · 2013Article
- Measurement of rate constants for homodimer subunit exchange using double electron-electron resonance and paramagnetic relaxation enhancements.Journal of biomolecular NMR · 2013Article
- Limiting assumptions in structure-based design: binding entropy.Journal of computer-aided molecular design · 2012Review
- Pulsed dipolar spectroscopy distance measurements in biomacromolecules labeled with Gd(III) markers.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2011Article
- EPR distance measurements in deuterated proteins.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2010Article
- Probing the (H3-H4)2 histone tetramer structure using pulsed EPR spectroscopy combined with site-directed spin labelling.Nucleic acids research · 2010Article
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Authors and funding
5 authors.
Funding
Abstract
The nucleosome core particle is the fundamental unit of chromatin structure and at its heart is the histone core octamer composed of histones H4, H3, H2A and H2B. To understand the structure dynamics and function of chromatin it is important to be able to probe the structures of its component parts in a variety of ways. Site directed spin-labeling technology has enabled the insertion of nitroxide spin labels at positions on the surface of the H3 histones and these have been assembled into histone octamers. Pulsed EPR, and in particular the PELDOR or DEER experiments have been performed and provided extremely well defined dipolar oscillations, over long time periods. From the PELDOR data we have been able to derive distance distributions of between 60 and 70 A. The distances measured, are among the longest well-defined PELDOR measurements on a biological system to date, spanning the width of the histone core particle and approaching what has been often defined as the limit of distance measurement by this technique. Relatively minor differences to the crystal structures have been observed.
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