ArticleInvestigative ophthalmology & visual science2009
Human corneal endothelial cell transplantation in a human ex vivo model.
Article in Investigative ophthalmology & visual science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 80 citations in OpenAlex.
- Establishment of an Ex Vivo Human Corneal Endothelium Wound Model.Translational vision science & technology · 2025Article
- A Framework for Human Corneal Endothelial Cell Culture and Preliminary Wound Model Experiments with a New Cell Tracking Approach.International journal of molecular sciences · 2023Article
- Corneal endothelial transplantation from bench to bedside: A review of animal models and their translational value for therapeutic development.Experimental eye research · 2022Review
- Evolution of therapies for the corneal endothelium: past, present and future approaches.The British journal of ophthalmology · 2021Review
- Fuchs endothelial corneal dystrophy: The vicious cycle of Fuchs pathogenesis.Progress in retinal and eye research · 2021Review
- Characterization of a dual media system for culturing primary normal and Fuchs endothelial corneal dystrophy (FECD) endothelial cells.PloS one · 2021Article
- Article
- Magnetic Human Corneal Endothelial Cell Transplant: Delivery, Retention, and Short-Term Efficacy.Investigative ophthalmology & visual science · 2019Article
- A Critical Review on Selected External Physical Cues and Modulation of Cell Behavior: Magnetic Nanoparticles, Non-thermal Plasma and Lasers.Journal of functional biomaterials · 2018Review
- Human Corneal Tissue Model for Nociceptive Assessments.Advanced healthcare materials · 2018Article
- Medical and Semi-surgical Treatments for Fuchs Endothelial Corneal Dystrophy.Klinische Monatsblatter fur Augenheilkunde · 2018Review
- A Cell Culture Approach to Optimized Human Corneal Endothelial Cell Function.Investigative ophthalmology & visual science · 2018Article
- From DMEK to Corneal Endothelial Cell Therapy: Technical and Biological Aspects.Journal of ophthalmology · 2018Review
- Wound-Healing Studies in Cornea and Skin: Parallels, Differences and Opportunities.International journal of molecular sciences · 2017Review
- Application of Rho Kinase Inhibitors for the Treatment of Corneal Endothelial Diseases.Journal of ophthalmology · 2017Review
- Novel Identity and Functional Markers for Human Corneal Endothelial Cells.Investigative ophthalmology & visual science · 2016Article
- In Vivo Functionality of a Corneal Endothelium Transplanted by Cell-Injection Therapy in a Feline Model.Investigative ophthalmology & visual science · 2016Article
- The pattern of early corneal endothelial cell recovery following cataract surgery: cellular migration or enlargement?Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2015Article
- Substrates for Expansion of Corneal Endothelial Cells towards Bioengineering of Human Corneal Endothelium.Journal of functional biomaterials · 2015Review
- Corneal stem cells and tissue engineering: Current advances and future perspectives.World journal of stem cells · 2015Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
purposeTo determine the effects of incorporating superparamagnetic microspheres (SPMs) into cultured human corneal endothelial cells (HCECs) and to describe preliminary experiments of HCEC transplantation, facilitated by SPMs and an external magnetic field, in a human anterior segment ex vivo model.
methodsHCECs were cultured as monolayers and incorporated with magnetite oxide SPMs (900, 300, and 100 nm) at different concentrations. Cell viability, migration toward a magnetic field, and light transmittance were measured after incorporation of the SPMs. HCEC transplantation into the eyes of human recipients was investigated by subjecting anterior segments in organ culture to an external magnetic field. Light and electron microscopy were used to assess HCEC attachment to corneal stroma.
resultsSPMs were incorporated into the cytoplasm of HCECs after overnight incubation. None of the SPMs affected the short-term viability of cultured HCECs (P > 0.14, n = 6) or their light transmittance (P > 0.06, n = 5), although there was a trend toward decreased transmittance with the higher concentration of 900-nm SPMs. Cell migration toward a magnetic field was higher for HCECs with incorporated SPMs than for HCECs without SPMs (P < or = 0.01, n = 6), with dose-response relationships evident for the 300- and 100-nm SPMs. SPMs facilitated the attachment of HCECs to the corneal stroma in the human anterior segment model with minimal change in intracameral (intraocular) pressure.
conclusionsSPMs facilitate migration of HCECs toward a magnetic source and attachment of cells to the corneal stroma without affecting cell viability or light transmittance. The human anterior segment model can be used to study HCEC transplantation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.