ArticleEukaryotic cell2009
Disruption of the Aopex11-1 gene involved in peroxisome proliferation leads to impaired Woronin body formation in Aspergillus oryzae.
Article in Eukaryotic cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 58 citations in OpenAlex.
- Expression pattern, subcellular localization ofApplied and environmental microbiology · 2025Article
- Woronin body hitchhiking on early endosomes is dispensable for septal localization inMolecular biology of the cell · 2023Article
- Large-scale identification of genes involved in septal pore plugging in multicellular fungi.Nature communications · 2023Article
- Woronin bodies move dynamically and bidirectionally by hitchhiking on early endosomes inbioRxiv : the preprint server for biology · 2023Article
- Identification of Six Thiolases and Their Effects on Fatty Acid and Ergosterol Biosynthesis inApplied and environmental microbiology · 2022Article
- Monitoring peroxisome dynamics using enhanced green fluorescent protein labeling inFrontiers in microbiology · 2022Article
- Aspergillus niger uses the peroxisomal CoA-dependent β-oxidative genes to degrade the hydroxycinnamic acids caffeic acid, ferulic acid, and p-coumaric acid.Applied microbiology and biotechnology · 2021Article
- Article
- Functional Genomics ofMicroorganisms · 2019Review
- Mevalonate Diphosphate Decarboxylase MVD/Erg19 Is Required for Ergosterol Biosynthesis, Growth, Sporulation and Stress Tolerance inFrontiers in microbiology · 2019Article
- Cell Biology of Hyphal Growth.Microbiology spectrum · 2017Review
- Switching from a unicellular to multicellular organization in an Aspergillus niger hypha.mBio · 2015Article
- Article
- A large nonconserved region of the tethering protein Leashin is involved in regulating the position, movement, and function of Woronin bodies in Aspergillus oryzae.Eukaryotic cell · 2014Article
- Peroxisome deficient invertebrate and vertebrate animal models.Frontiers in physiology · 2013Review
- The HEX1 gene of Fusarium graminearum is required for fungal asexual reproduction and pathogenesis and for efficient viral RNA accumulation of Fusarium graminearum virus 1.Journal of virology · 2013Article
- Aspergillus oryzae AoSO is a novel component of stress granules upon heat stress in filamentous fungi.PloS one · 2013Article
- Expanding functional repertoires of fungal peroxisomes: contribution to growth and survival processes.Frontiers in physiology · 2013Article
- A newly discovered function of peroxisomes: involvement in biotin biosynthesis.Plant signaling & behavior · 2012Review
- Integration of peroxisomes into an endomembrane system that governs cellular aging.Frontiers in physiology · 2012Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Woronin body, a unique organelle found in the Pezizomycotina, plugs the septal pore upon hyphal damage to prevent excessive cytoplasmic bleeding. Although it was previously shown that the Woronin body buds out from the peroxisome, the relationship between peroxisomal proliferation/division and Woronin body differentiation has not been extensively investigated. In this report, we examined whether Pex11 required for peroxisomal proliferation participates in Woronin body formation in Aspergillus oryzae. A. oryzae contained two orthologous PEX11 genes that were designated Aopex11-1 and Aopex11-2. Deletion of Aopex11 genes revealed that only the DeltaAopex11-1 strain showed reduced growth and enlarged peroxisomes in the presence of oleic acid as a sole carbon source, indicating a defect in peroxisomal function and proliferation. Disruption of Aopex11-1 gene impaired the Woronin body function, leading to excessive loss of the cytosol upon hyphal injury. Dual localization analysis of the peroxisome and Woronin body protein AoHex1 demonstrated that Woronin bodies fail to fully differentiate from peroxisomes in the DeltaAopex11-1 strain. Furthermore, distribution of AoHex1 was found to be peripheral in the enlarged peroxisome or junctional in dumbbell-shaped peroxisomes. Electron microscopy of the DeltaAopex11-1 strain revealed the presence of Woronin bodies that remained associated with organelles resembling peroxisomes, which was supported from the sucrose gradient centrifugation confirming that the Woronin body protein AoHex1 overlapped with the density-shifted peroxisome in the DeltaAopex11-1 strain. In conclusion, the present study describes the role of Pex11 in Woronin body differentiation for the first time.
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