ArticlePlant physiology2009
Tyrosine and phenylalanine are synthesized within the plastids in Arabidopsis.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
59 citing papers in PubMed, 159 citations in OpenAlex.
- Article
- Salicylic acid-mediated competitive regulation of xylem vessel formation and defense responses in Arabidopsis thaliana.Plant & cell physiology · 2025Article
- RETICULATA1 is a plastid-localized basic amino acid transporter.Nature plants · 2025Article
- Article
- Gene Localization and Functional Validation ofPlants (Basel, Switzerland) · 2025Article
- Maize big embryo 6 reveals roles of plastidial and cytosolic prephenate aminotransferases in seed and plant development.The Plant cell · 2025Article
- GhADT5 enhances alkali stress tolerance in cotton by regulating phenylalanine-derived flavonoid biosynthesis and antioxidant defense.BMC plant biology · 2025Article
- Arabidopsis 3-Deoxy-d-Plant direct · 2025Article
- Insights into physiological roles of flavonoids in plant cold acclimation.The Plant journal : for cell and molecular biology · 2024Article
- A simplified liquid chromatography-mass spectrometry methodology to probe the shikimate and aromatic amino acid biosynthetic pathways in plants.The Plant journal : for cell and molecular biology · 2024Article
- Evolution of aromatic amino acid metabolism in plants: a key driving force behind plant chemical diversity in aromatic natural products.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- The cytosolic aminotransferase VAS1 coordinates aromatic amino acid biosynthesis and metabolism.Science advances · 2024Article
- Soybean AROGENATE DEHYDRATASES (GmADTs): involvement in the cytosolic isoflavonoid metabolon or trans-organelle continuity?Frontiers in plant science · 2024Article
- Expression Patterns and Functional Analysis of ThreeInternational journal of molecular sciences · 2023Article
- The Sapria himalayana genome provides new insights into the lifestyle of endoparasitic plants.BMC biology · 2023Article
- Xanthones: Biosynthesis and Trafficking in Plants, Fungi and Lichens.Plants (Basel, Switzerland) · 2023Review
- Arogenate dehydratases: unique roles in light-directed development during the seed-to-seedling transition inFrontiers in plant science · 2023Article
- Deregulation of phenylalanine biosynthesis evolved with the emergence of vascular plants.Plant physiology · 2022Article
- Floral Scents and Fruit Aromas: Functions, Compositions, Biosynthesis, and Regulation.Frontiers in plant science · 2022Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While the presence of a complete shikimate pathway within plant plastids is definitively established, the existence of a cytosolic postchorismate portion of the pathway is still debated. This question is alimented by the presence of a chorismate mutase (CM) within the cytosol. Until now, the only known destiny of prephenate, the product of CM, is incorporation into tyrosine (Tyr) and/or phenylalanine (Phe). Therefore, the presence of a cytosolic CM suggests that enzymes involved downstream of CM in Tyr or Phe biosynthesis could be present within the cytosol of plant cells. It was thus of particular interest to clarify the subcellular localization of arogenate dehydrogenases (TYRAs) and arogenate dehydratases (ADTs), which catalyze the ultimate steps in Tyr and Phe biosynthesis, respectively. The aim of this study was to address this question in Arabidopsis (Arabidopsis thaliana) by analysis of the subcellular localization of the two TYRAAts and the six AtADTs. This article excludes the occurrence of a spliced TYRAAt1 transcript encoding a cytosolic TYRA protein. Transient expression analyses of TYRA- and ADT-green fluorescent protein fusions reveal that the two Arabidopsis TYRA proteins and the six ADT proteins are all targeted within the plastid. Accordingly, TYRA and ADT proteins were both immunodetected in the chloroplast soluble protein fraction (stroma) of Arabidopsis. No TYRA or ADT proteins were immunodetected in the cytosol of Arabidopsis cells. Taken together, all our data exclude the possibility of Tyr and/or Phe synthesis within the cytosol, at least in green leaves and Arabidopsis cultured cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.