ArticleBiophysical journal2009
Backbone structure of transmembrane domain IX of the Na+/proline transporter PutP of Escherichia coli.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Advances in EPR Approaches for Studying Structural Properties of Membrane Proteins.Magnetic resonance in chemistry : MRC · 2026Review
- Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana.Scientific reports · 2022Article
- Genomic Analysis ofFrontiers in microbiology · 2021Article
- Electron Paramagnetic Resonance as a Tool for Studying Membrane Proteins.Biomolecules · 2020Review
- Comparison of the functional properties of trimeric and monomeric CaiT of Escherichia coli.Scientific reports · 2019Article
- Site-Directed Spin Labeling EPR for Studying Membrane Proteins.BioMed research international · 2018Review
- Relative Orientation of POTRA Domains from Cyanobacterial Omp85 Studied by Pulsed EPR Spectroscopy.Biophysical journal · 2016Article
- Glu-311 in External Loop 4 of the Sodium/Proline Transporter PutP Is Crucial for External Gate Closure.The Journal of biological chemistry · 2016Article
- Doubly Spin-Labeled RNA as an EPR Reporter for Studying Multicomponent Supramolecular Assemblies.Biophysical journal · 2015Article
- Investigating the structure of the factor B vWF-A domain/CD55 protein-protein complex using DEER spectroscopy: successes and pitfalls.Molecular physics · 2013Article
- DEER EPR measurements for membrane protein structures via bifunctional spin labels and lipodisq nanoparticles.Biochemistry · 2013Article
- Use of electron paramagnetic resonance to solve biochemical problems.Biochemistry · 2013Review
- A comparative study of structures and structural transitions of secondary transporters with the LeuT fold.European biophysics journal : EBJ · 2013Article
- Article
- Conformational state of the MscS mechanosensitive channel in solution revealed by pulsed electron-electron double resonance (PELDOR) spectroscopy.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The global analysis of DEER data.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2012Article
- Simulating the dynamics and orientations of spin-labeled side chains in a protein-DNA complex.The journal of physical chemistry. B · 2012Article
- Rigid core and flexible terminus: structure of solubilized light-harvesting chlorophyll a/b complex (LHCII) measured by EPR.The Journal of biological chemistry · 2012Article
- Toward the fourth dimension of membrane protein structure: insight into dynamics from spin-labeling EPR spectroscopy.Structure (London, England : 1993) · 2011Review
- Algorithm for selection of optimized EPR distance restraints for de novo protein structure determination.Journal of structural biology · 2011Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The backbone structure is determined by site-directed spin labeling, double electron electron resonance measurements of distances, and modeling in terms of a helix-loop-helix construct for a transmembrane domain that is supposed to line the translocation pathway in the 54.3 kDa Na(+)/proline symporter PutP of Escherichia coli. The conformational distribution of the spin labels is accounted for by a rotamer library. An ensemble of backbone models with a root mean-square deviation of less than 2 A is obtained. These models exhibit a pronounced kink near residue T341, which is involved in substrate binding. The kink may be associated with a hinge that allows the protein to open and close an inwardly oriented cavity.
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