ArticleBiophysical journal2009
Simultaneous measurement of nucleotide occupancy and mechanical displacement in myosin-V, a processive molecular motor.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Sarcomere length nanometry in rat neonatal cardiomyocytes expressed with α-actinin-AcGFP in Z discs.The Journal of general physiology · 2014Article
- Single-molecule fluorescence imaging of processive myosin with enhanced background suppression using linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC).Optics express · 2013Article
- Future challenges in single-molecule fluorescence and laser trap approaches to studies of molecular motors.Developmental cell · 2012Review
- G146V mutation at the hinge region of actin reveals a myosin class-specific requirement of actin conformations for motility.The Journal of biological chemistry · 2012Article
- Myosin-V as a mechanical sensor: an elastic network study.Biophysical journal · 2012Article
- Simple dark-field microscopy with nanometer spatial precision and microsecond temporal resolution.Biophysical journal · 2010Article
- Watching the walk: observing chemo-mechanical coupling in a processive myosin motor.HFSP journal · 2009Article
Corrections and comments
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Authors and funding
5 authors.
Funding
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Abstract
Adenosine triphosphate (ATP) turnover drives various processive molecular motors and adenosine diphosphate (ADP) release is a principal transition in this cycle. Biochemical and single molecule mechanical studies have led to a model in which a slow ADP release step contributes to the processivity of myosin-V. To test the relationship between force generation and ADP release, we utilized optical trapping nanometry and single molecule total internal reflection fluorescence imaging for simultaneous and direct observation of both processes in myosin-V. We found that ADP was released 69 +/- 5.3 ms after force generation and displacement of actin, providing direct evidence for slow ADP release. As proposed by several previous studies, this slow ADP release probably ensures processivity by prolonging the strong actomyosin state in the ATP turnover cycle.
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Registered trials
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