Evidence map›Paper›PMID 19134467›Full record

ArticleBiophysical journal2009

In vivo labeling method using a genetic construct for nanoscale resolution microscopy.

Jan Schröder, Hélène Benink, Marcus Dyba, Georgyi V Los

Abstract read
In one paragraph

Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Review
  2. Single-Molecule Imaging in Living Plant Cells: A Methodological Review.International journal of molecular sciences · 2021
    Review
  3. Visualization of integrin molecules by fluorescence imaging and techniques.Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al · 2021
    Article
  4. Review
  5. STAQ: A route toward low power, multicolor nanoscopy.Microscopy research and technique · 2015
    Article
  6. Review
  7. Fluorescence nanoscopy. Methods and applications.Journal of chemical biology · 2013
    Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. HaloTag: a novel reporter gene for positron emission tomography.American journal of translational research · 2011
    Article
  16. A guide to super-resolution fluorescence microscopy.The Journal of cell biology · 2010
    Review
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jan SchröderLeica Microsystems CMS, Mannheim, Germany. jan.schroeder@leica-microsystems.com
Hélène Benink
Marcus Dyba
Georgyi V Los

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We demonstrate beam scanning-stimulated emission depletion microscopy with in vivo labeled cells. A red emitting fluorescent dye is introduced into membrane protein fused to a multifunctional reporter protein (HaloTag, Promega, Madison, WI) in live cells. This approach allows superresolution stimulated emission depletion imaging without the limitations of immunofluorescence-based staining.

Indexed as

Gene Transfer TechniquesCell Line, TumorFluorescent DyesGenes, ReporterHeLa CellsHumansIntegrin beta1MicroscopyPseudopodiaFluorescent DyesIntegrin beta1

Identifiers

PMID19134467
PMCPMC2710050

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.